BMRB Entry 6741

Title:
NMR Structure of Mini-B, an N-terminal - C-terminal construct from Surfactant Protein B (SP-B), in Sodium Dodecyl Sulfate (SDS) and Hexafluoroisopropanol (HFIP)   PubMed: 17845058
Deposition date:
2005-07-21
Original release date:
2007-11-14
Authors:
Booth, V.; Sarker, M.; Keough, K.; Waring, A.; Walther, F.
Citation:

Citation: Sarker, M.; Waring, A.; Walther, F.; Keough, K.; Booth, V.. "Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles"  Biochemistry 46, 11047-11056 (2007).

Assembly members:

Assembly members:
Pulmonary surfactant-associated protein B, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pulmonary surfactant-associated protein B: CWLCRALIKRIQAMIPKGGR MLPQLVCRLVLRCS

Data sets:
Data typeCount
1H chemical shifts554

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pulmonary surfactant-associated protein B1

Entities:

Entity 1, Pulmonary surfactant-associated protein B 34 residues - Formula weight is not available

1   CYSTRPLEUCYSARGALALEUILELYSARG
2   ILEGLNALAMETILEPROLYSGLYGLYARG
3   METLEUPROGLNLEUVALCYSARGLEUVAL
4   LEUARGCYSSER

Samples:

sample_1: Pulmonary surfactant-associated protein B, [15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18), 1.5 mM; SDS 150 mM; DSS 0.4 mM; NaN3 0.2 mM; H2O 90%; D2O 10%

sample_2: Pulmonary surfactant-associated protein B, [15N]-Leu(3,7,22,25,29,31), Ala(6,13), and Gly(18), 1.5 mM; HFIP 40 mM; DSS 0.4 mM; NaN3 0.2 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5; temperature: 318 K; ionic strength: 0 M; pressure: 1 atm

sample_cond_2: pH: 5; temperature: 288 K; ionic strength: 0 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available

Software:

NMRPipe v2.2 - processing

Sparky v3.110 - data analysis

CNS v1.1 - refinement

MOLMOL v2K.2 - data analysis

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB