BMRB Entry 6740

Name: NMR solution structure of the Crisp domain of Tpx-1
Published: 2007-02-06

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PDB ID: 2a05
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6740
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of the Crisp domain of Tpx-1

Deposition date:

2005-07-21

Original release date:

2007-02-06

Authors:

Gibbs, G.; Scanlon, M.; Swarbrick, J.; Curtis, S.; Dulhunty, A.; O'Bryan, M.

Citation:

Citation: Gibbs, G.; Scanlon, M.; Swarbrick, J.; Curtis, S.; Gallant, E.; Dulhunty, A.; O'Bryan, M.. "The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signalling" J. Biol. Chem. 281, 4156-4163 (2006).
PubMed: 16339766

Assembly members:

Assembly members:
Cysteine-rich secretory protein-2, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cysteine-rich secretory protein-2: GSCASCPNNCENGLCTNSCD FEDLLSNCESLKTSAGCKHE LLKTKCQATCLCEDKIH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	78
15N chemical shifts	58
1H chemical shifts	293

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cysteine-rich secretory protein-2	1

Entities:

Entity 1, Cysteine-rich secretory protein-2 57 residues - Formula weight is not available

1

GLY

SER

CYS

ALA

SER

CYS

PRO

ASN

CYS

2

GLU

ASN

GLY

LEU

CYS

THR

ASN

SER

CYS

ASP

3

PHE

GLU

ASP

LEU

SER

ASN

CYS

GLU

SER

4

LEU

LYS

THR

SER

ALA

GLY

CYS

LYS

HIS

GLU

5

LEU

LYS

THR

LYS

CYS

GLN

ALA

THR

CYS

6

LEU

CYS

GLU

ASP

LYS

ILE

HIS

Samples:

sample_1: Cysteine-rich secretory protein-2, [U-13C; U-15], 1 mM; H2O 90%; D2O 10%

sample_2: Cysteine-rich secretory protein-2 1 mM; H2O 90%; D2O 10%

sample_3: Cysteine-rich secretory protein-2, [U-13C; U-15], 1 mM; D2O 100%

sample_cond_1: ionic strength: 1 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	not available	not available	not available
3D_15N-separated TOCSY	not available	not available	not available
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available

Software:

XWINNMR v1, Bruker - collection, processing

SPARKY v3.101, Goddard Keller - data analysis

CYANA v1.06, Guntert - refinement

XPLOR-NIH v2.9.9, Clore Schwieters Kuszewski Tjandra - refinement

NMR spectrometers:

Bruker DRX 500 MHz

PDB	2A05
GenBank	AAA40472 AAH49615 EDL23385
REF	NP_033446
SWISS-PROT	P16563