BMRB Entry 6738

Name: Solution structure of Sep15 from Drosophila melanogaster
Published: 2005-12-28

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PDB ID: 2a4h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6738
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Sep15 from Drosophila melanogaster

Deposition date:

2005-07-21

Original release date:

2005-12-28

Authors:

Ferguson, A.; Labunskyy, V.; Fomenko, D.; Arac, D.; Chelliah, Y.; Amezcua, C.; Rizo, J.; Gladyshev, V.; Deisenhofer, J.

Citation:

Citation: Ferguson, A.; Labunskyy, V.; Fomenko, D.; Arac, D.; Chelliah, Y.; Amezcua, C.; Rizo, J.; Gladyshev, V.; Deisenhofer, J.. "NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of new thioredoxin-like family." J. Biol. Chem. ., .-. (2005).
PubMed: 16319061

Assembly members:

Assembly members:
Selenoprotein Sep15, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Drosophila melanogaster Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Selenoprotein Sep15: MASHHHHHHLDQQPAAQRTY AKAILEVCTCKFRAYPQIQA FIQSGRPAKFPNLQIKYVRG LDPVVKLLDASGKVQETLSI TKWNTDTVEEFFETHLAKDG AGKNSYSVVEDADGDDDEDY LRTNRI

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	809
13C chemical shifts	496
15N chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Selenoprotein Sep15	1

Entities:

Entity 1, Selenoprotein Sep15 126 residues - Formula weight is not available

1

MET

ALA

SER

HIS

LEU

2

ASP

GLN

PRO

ALA

GLN

ARG

THR

TYR

3

ALA

LYS

ALA

ILE

LEU

GLU

VAL

CYS

THR

CYS

4

LYS

PHE

ARG

ALA

TYR

PRO

GLN

ILE

GLN

ALA

5

PHE

ILE

GLN

SER

GLY

ARG

PRO

ALA

LYS

PHE

6

PRO

ASN

LEU

GLN

ILE

LYS

TYR

VAL

ARG

GLY

7

LEU

ASP

PRO

VAL

LYS

LEU

ASP

ALA

8

SER

GLY

LYS

VAL

GLN

GLU

THR

LEU

SER

ILE

9

THR

LYS

TRP

ASN

THR

ASP

THR

VAL

GLU

10

PHE

GLU

THR

HIS

LEU

ALA

LYS

ASP

GLY

11

ALA

GLY

LYS

ASN

SER

TYR

SER

VAL

GLU

12

ASP

ALA

ASP

GLY

ASP

GLU

ASP

TYR

13

LEU

ARG

THR

ASN

ARG

ILE

Samples:

sample_1: Selenoprotein Sep15, [U-15N; U-13C], 1 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY	sample_1	not available	sample_cond_1
2D NOESY	sample_1	not available	sample_cond_1
2D TOCSY	sample_1	not available	sample_cond_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v1, Delaglio - processing

NMRView v5.2.2, Johnson - data analysis

ARIA v2.0, Nilges - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz

REF	XP_002094982 XP_002076553 XP_002030922 NP_649000
GenBank	EDX15740 EDW94694 EDW41908 AAM51078 AAF49314
EMBL	CAL26591 CAL26585 CAL26581 CAL26580 CAL26579
PDB	2A4H