BMRB Entry 6736

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6736

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Pseudomonas Aeruginosa Protein Pa2021. The Northeast Structural Genomics Consortium Target Pat85.
Deposition date:
2005-07-20
Original release date:
2005-10-20
Authors:
Lin, Yu-Chieh; Liu, Gaohua; Shen, Yang; Yee, Adelinda; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Lin, Yu-Chieh; Liu, Gaohua; Shen, Yang; Bertonati, C.; Yee, Adelinda; Honig, B.; Arrowsmith, Cheryl; Szyperski, Thomas. "NMR structure of protein PA2021 from Pseudomonas aeruginosa"  Proteins 65, 767-770 (2006).
PubMed: 16927296

Assembly members:

Assembly members:
PA2021, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PA2021: MGTSHHHHHHSSGRENLYFQ GHMSIEIDSEQGVCSVEIEG SRHRAPVDSLRIGTDAEARL SVLYIDGKRLHISEEDAQRL VVAGAEDQRRHLMADD

Data sets:
Data typeCount
13C chemical shifts206
15N chemical shifts71
1H chemical shifts489

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NMR structure of PA20211

Entities:

Entity 1, NMR structure of PA2021 96 residues - Formula weight is not available

1   METGLYTHRSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETSERILEGLUILEASPSERGLU
4   GLNGLYVALCYSSERVALGLUILEGLUGLY
5   SERARGHISARGALAPROVALASPSERLEU
6   ARGILEGLYTHRASPALAGLUALAARGLEU
7   SERVALLEUTYRILEASPGLYLYSARGLEU
8   HISILESERGLUGLUASPALAGLNARGLEU
9   VALVALALAGLYALAGLUASPGLNARGARG
10   HISLEUMETALAASPASP

Samples:

PA2021: PA2021, [U-13C; U-15N], 0.6 mM

solution: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCPA2021not availablesolution
1H13C HSQCPA2021not availablesolution
CBCAcoNHPA2021not availablesolution
HNCACBPA2021not availablesolution
HNCAPA2021not availablesolution
CAcoNHPA2021not availablesolution
GFT-HNCAHAPA2021not availablesolution
GFT-HCCHCOSYPA2021not availablesolution

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAK89844 BAP23545 BAP51149 GAA17576 GAJ53932
EMBL CAW28029 CCQ85418 CDH71317 CDH77600 CDI90261
GB AAG05409 ABJ11207 AEO75506 AFM65387 AGI81918
REF NP_250711 WP_003088627 WP_003100372 WP_003120203 WP_014602995

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks