BMRB Entry 6720

Title:
Solution Conformation of alpha-conotoxin PIA
Deposition date:
2005-06-30
Original release date:
2008-07-16
Authors:
Chi, S.-W.; Kim, D.-H.; Olivera, B.; Mclntosh, J.; Han, K.-H.
Citation:

Citation: Chi, S.-W.; Lee, S.-H.; Kim, D.-H.; Kim, J.-S.; Olivera, B.; Mclntosh, J.; Han, K.-H.. "Solution structure of alpha-conotoxin PIA, a novel antagonist of alpha6 subunit containing nicotinic acetylcholine receptors"  Biochem. Biophys. Res. Commun. 338, 1990-1997 (2005).
PubMed: 16289101

Assembly members:

Assembly members:
Alpha-conotoxin PIA, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Conus purpurascens   Taxonomy ID: 41690   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus purpurascens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alpha-conotoxin PIA: RDPCCSNPVCTVHNPQICX

Data sets:
Data typeCount
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha-conotoxin PIA1

Entities:

Entity 1, Alpha-conotoxin PIA 19 residues - Formula weight is not available

1   ARGASPPROCYSCYSSERASNPROVALCYS
2   THRVALHISASNPROGLNILECYSNH2

Samples:

sample_1: Alpha-conotoxin PIA 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0 M; pH: 3.92; pressure: 1 atm; temperature: 283 K

sample_cond_2: ionic strength: 0 M; pH: 3.92; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablenot available
2D TOCSYsample_1not availablenot available
DQF-COSYsample_1not availablenot available

Software:

VNMR v6,1B - collection

FELIX v98.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
SWISS-PROT P69658