BMRB Entry 6715

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6715
MolProbity Validation Chart

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Title:
Solution NMR Structure of the Membrane Protein Stannin
Deposition date:
2005-06-30
Original release date:
2007-01-29
Authors:
Buck-Koehntop, B.; Mascioni, A.; Buffy, J.; Veglia, G.
Citation:

Citation: Buck-Koehntop, B.; Mascioni, A.; Buffy, J.; Veglia, G.. "Structure, Dynamics, and Membrane Topology of Stannin: A Mediator of Neuronal Cell Apoptosis Induced by Trimethyltin Chloride."  J. Mol. Biol. 354, 652-665 (2005).
PubMed: 16246365

Assembly members:

Assembly members:
Stannin, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Stannin: GSMSIMDHSPTTGVVTVIVI LIAIAALGALILGCWCYLRL QRISQSEDEESIVGDGETKE PFLLVQYSAKGPCVERKAKL MTPNGPEVHG

Data sets:
Data typeCount
1H chemical shifts165
13C chemical shifts170
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Stannin1

Entities:

Entity 1, Stannin 90 residues - Formula weight is not available

1   GLYSERMETSERILEMETASPHISSERPRO
2   THRTHRGLYVALVALTHRVALILEVALILE
3   LEUILEALAILEALAALALEUGLYALALEU
4   ILELEUGLYCYSTRPCYSTYRLEUARGLEU
5   GLNARGILESERGLNSERGLUASPGLUGLU
6   SERILEVALGLYASPGLYGLUTHRLYSGLU
7   PROPHELEULEUVALGLNTYRSERALALYS
8   GLYPROCYSVALGLUARGLYSALALYSLEU
9   METTHRPROASNGLYPROGLUVALHISGLY

Samples:

sample_1: Stannin, [U-15N], 0.5 – 0.7 mM; phosphate buffer 20 mM; d25-sodium dodecyl sulfate 600 mM; sodium azide 0.02%; DTT 0.7 mM; H2O 90%; D2O 10%

sample_2: Stannin, [U-13C; U-15N], 0.5 – 0.7 mM; phosphate buffer 20 mM; d25-sodium dodecyl sulfate 600 mM; sodium azide 0.02%; DTT 0.7 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.0; temperature: 333 K; ionic strength: 120 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1c - collection

NMRPipe v97.027.12.56 - processing

Sparky v3.111 - data analysis

X-PLOR v2.11 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE29024 BAI46912
EMBL CAB82314 CAG46924 CAG46935 CAL37610
GB AAC28427 AAC28428 AAC83231 AAH06961 AAH17685
REF NP_001029255 NP_001107194 NP_001180368 NP_003489 NP_033249
SP O75324 P61807 P61808 Q17Q87
TPG DAA15615
AlphaFold O75324 P61807 P61808 Q17Q87

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks