BMRB Entry 6711

Name: NMR assignment of Human Ubiquitin Conjugating Enzyme (Ubc7)
Published: 2005-10-26

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6711

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of Human Ubiquitin Conjugating Enzyme (Ubc7)

Deposition date:

2005-06-30

Original release date:

2005-10-26

Authors:

Briggman, Kathryn; Majumdar, Ananya; Coleman, Catherine; Chau, Vincent; Tolman, Joel

Citation:

Citation: Briggman, Kathryn; Majumdar, Ananya; Coleman, Catherine; Chau, Vincent; Tolman, Joel. "NMR Assignment of Human Ubiquitin Conjugating Enzyme Ubc7" J. Biomol. NMR 32, 340-340 (2005).
PubMed: 16211496

Assembly members:

Assembly members:
hUbc7, polymer, 165 residues, 18504 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapien

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hUbc7: MAGTALKRLMAEYKQLTLNP PEGIVAGPMNEENFFEWEAL IMGPEDTCFEFGVFPAILSF PLDYPLSPPKMRFTCEMFHP NIYPDGRVCISILHAPGDDP MGYESSAERWSPVQSVEKIL LSVVSMLAEPNDESGANVDA SKMWRDDREQFYKIAKQIVQ KSLGL

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	645
15N chemical shifts	147
1H chemical shifts	923

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubc7	1

Entities:

Entity 1, Ubc7 165 residues - 18504 Da.

1

MET

ALA

GLY

THR

ALA

LEU

LYS

ARG

LEU

MET

2

ALA

GLU

TYR

LYS

GLN

LEU

THR

LEU

ASN

PRO

3

PRO

GLU

GLY

ILE

VAL

ALA

GLY

PRO

MET

ASN

4

GLU

ASN

PHE

GLU

TRP

GLU

ALA

LEU

5

ILE

MET

GLY

PRO

GLU

ASP

THR

CYS

PHE

GLU

6

PHE

GLY

VAL

PHE

PRO

ALA

ILE

LEU

SER

PHE

7

PRO

LEU

ASP

TYR

PRO

LEU

SER

PRO

LYS

8

MET

ARG

PHE

THR

CYS

GLU

MET

PHE

HIS

PRO

9

ASN

ILE

TYR

PRO

ASP

GLY

ARG

VAL

CYS

ILE

10

SER

ILE

LEU

HIS

ALA

PRO

GLY

ASP

PRO

11

MET

GLY

TYR

GLU

SER

ALA

GLU

ARG

TRP

12

SER

PRO

VAL

GLN

SER

VAL

GLU

LYS

ILE

LEU

13

LEU

SER

VAL

SER

MET

LEU

ALA

GLU

PRO

14

ASN

ASP

GLU

SER

GLY

ALA

ASN

VAL

ASP

ALA

15

SER

LYS

MET

TRP

ARG

ASP

ARG

GLU

GLN

16

PHE

TYR

LYS

ILE

ALA

LYS

GLN

ILE

VAL

GLN

17

LYS

SER

LEU

GLY

LEU

Samples:

sample_1: hUbc7, [U-13C; U-15N], 1 mM; Tris buffer 50 mM; TCEP 2 mM; NaN3 0.05%; D2O 10%

sample_2: hUbc7, [U-2H; U-13C; U-15N], 1 mM; Tris buffer 10 mM; TCEP 2 mM; NaN3 0.05%; D2O 10%

conditions_1: ionic strength: 50 mM; pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
HN(CA)CO	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HMQC-NOESY-HSQC	sample_1	not available	conditions_1
HBHA(CO)NH	sample_2	not available	conditions_1
H(CCCO)NH-TOCSY	sample_1	not available	conditions_1
CC(CO)NH-TOCSY	sample_2	not available	conditions_1
(H)CC(CO)NH-TOCSY	sample_1	not available	conditions_1
15N-edited_NOESY-HSQC	sample_1	not available	conditions_1

Software:

NMRView, One Moon Scientific, Inc. - assignment analysis

NMRPipe, NIH - data processing

NMR spectrometers:

Bruker Avance 600 MHz
Varian Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks