BMRB Entry 6710
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6710
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Citation: Chenavas, Sylvie; Simorre, Jean-pierre; Dubosclard, Virginie; Pebay-peyroula, Eva; Gans, Pierre; Brutscher, Bernhard; Fieschi, Franck. "NMR characterization of free and complexed p67phox and p40phox PB1 domains in solution" Eur. J. Biochem. ., .-..
Assembly members:
PB1 p67, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PB1 p67: MGSSHHHHHHSSGLVPRGSH
MKLSVPMPYTLKVHYKYTVV
MKTQPGLPYSQVRDMVSKKL
ELRLEHTKLSYRPRDSNELV
PLSEDSMKDAWGQVKNYCLT
LWCEN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 220 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PB1 domain of p67phox | 1 |
Entities:
Entity 1, PB1 domain of p67phox 105 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LYS | LEU | SER | VAL | PRO | MET | PRO | TYR | THR | ||||
| 4 | LEU | LYS | VAL | HIS | TYR | LYS | TYR | THR | VAL | VAL | ||||
| 5 | MET | LYS | THR | GLN | PRO | GLY | LEU | PRO | TYR | SER | ||||
| 6 | GLN | VAL | ARG | ASP | MET | VAL | SER | LYS | LYS | LEU | ||||
| 7 | GLU | LEU | ARG | LEU | GLU | HIS | THR | LYS | LEU | SER | ||||
| 8 | TYR | ARG | PRO | ARG | ASP | SER | ASN | GLU | LEU | VAL | ||||
| 9 | PRO | LEU | SER | GLU | ASP | SER | MET | LYS | ASP | ALA | ||||
| 10 | TRP | GLY | GLN | VAL | LYS | ASN | TYR | CYS | LEU | THR | ||||
| 11 | LEU | TRP | CYS | GLU | ASN |
Samples:
15N_PB1_p67: PB1 p67, [U-98% 15N], 1.2 mM; HEPES 50 mM; NaCl 100 mM; Dithiothreitol 2 mM
15N_13C_PB1_p67: PB1 p67, [U-98% 15N; U-97% 13C], 1.1 mM; HEPES 50 mM; NaCl 100 mM; Dithiothreitol 2 mM
conditions_1: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-HNCA | not available | not available | not available |
| 2D-HN(CO)CA | not available | not available | not available |
| 2D-HN(CA)CB | not available | not available | not available |
| 2D-HN(COCA)CB | not available | not available | not available |
| 2D-HN(CA)HA | not available | not available | not available |
| 2D-HN(COCA)HA | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| DBJ | BAD92884 BAG35548 BAG59269 BAG60869 BAJ20290 |
| GB | AAA36379 AAB60320 AAH01606 AAM89263 AAP36107 |
| REF | NP_000424 NP_001121123 NP_001177718 NP_001177723 XP_004028077 |
| SP | P19878 |
| AlphaFold | P19878 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks