BMRB Entry 6693

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6693

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Title:
Structure Determination of the Northeast Structural Genomics Consortium Target BoR11
Deposition date:
2005-06-16
Original release date:
2006-04-06
Authors:
Rossi, Paolo
Citation:

Citation: Rossi, Paolo; Ramelot, Theresa; Xiao, Rong; Ho, Chi; Ma, Li-Chung; Acton, Thomas; Kennedy, Michael; Montelione, Gaetano. "1H, 13C, and 15N Resonance Assignments for the Protein Coded by Gene Locus BB0938 of Bordetella bronchiseptica"  J. Biomol. NMR 33, 197-197 (2005).
PubMed: 16331425

Assembly members:

Assembly members:
BoR11, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bordetella bronchiseptica   Taxonomy ID: 1063   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Bordetella bronchiseptica

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pet21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BoR11: MSTTAYQPIAECGATTQSEA AAYQKRWLVANDAGQWLNRD LCPRLAEVSVELRMGYLVLK APGMLRLDIPLDVIEDDDSV RYQMLVGEQTVDVVDEGELA AAWISNHAGVPCRILKVHPD MAEVRWPSLEHHHHHH

Data typeCount
13C chemical shifts559
15N chemical shifts140
1H chemical shifts904

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BoR111

Entities:

Entity 1, BoR11 136 residues - Formula weight is not available

1   METSERTHRTHRALATYRGLNPROILEALA
2   GLUCYSGLYALATHRTHRGLNSERGLUALA
3   ALAALATYRGLNLYSARGTRPLEUVALALA
4   ASNASPALAGLYGLNTRPLEUASNARGASP
5   LEUCYSPROARGLEUALAGLUVALSERVAL
6   GLULEUARGMETGLYTYRLEUVALLEULYS
7   ALAPROGLYMETLEUARGLEUASPILEPRO
8   LEUASPVALILEGLUASPASPASPSERVAL
9   ARGTYRGLNMETLEUVALGLYGLUGLNTHR
10   VALASPVALVALASPGLUGLYGLULEUALA
11   ALAALATRPILESERASNHISALAGLYVAL
12   PROCYSARGILELEULYSVALHISPROASP
13   METALAGLUVALARGTRPPROSERLEUGLU
14   HISHISHISHISHISHIS

Samples:

sample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004: BoR11, [U-100% 13C; U-100% 15N], 0.7 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%

conditions_1: ionic strength: 0.1 M; pH: 6.5; temperature: 293 K

conditions_2: ionic strength: 0.1 M; pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
hCBCAcoNHsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HcccoNH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNCAsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNCACBsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNCOsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNcoCAsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
13C-edited NOESYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCcoNH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HcccoNH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCH-COSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
CH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
CH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
CH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
15N-edited NOESYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
CH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
13C-edited NOESYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNHAsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HBHAcbcacoNHsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HBHAcbcaNHsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HNCACOsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
NH_HSQCsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
H_COBBsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available
HCCH-TOCSYsample_BoR11.BoR11-21.3-NC-GF-6.5_BoR11.004not availablenot available

Software:

vnmr - data collection

xwin-nmr - data collection

NmrPipe - raw spectral data processing

Sparky - spectral visualization

AutoAssign - automated backbone assignments

AutoStructure - automated structural determination

SPINS - integrated spectral analysis software

NMR spectrometers:

  • varian null 750 MHz
  • bruker null 500 MHz
  • varian UNITY 600 MHz
  • varian UNITY Inova 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks