BMRB Entry 6689
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6689
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Yang, Ao; Miron, Simona; Duchambon, Patricia; Assairi, Liliane; Blouquit, Yves; Craescu, Constantin. "The N-terminal domain of human centrin 2 has a closed structure, binds calcium with a very low
affinity, and plays a role in the protein self-assembly" Biochemistry 45, 880-889 (2006).
PubMed: 16411764
Assembly members:
N-HsCen1, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-HsCen1: MASGFKKPSAASTGQKRKVA
PKPELTEDQKQEVREAFDLF
DVDGSGTIDAKELKVAMRAL
GFEPRKEEMKKMISEVDREG
TGKISFNDFLAVMTQKM
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 533 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human centrin 1 | 1 |
Entities:
Entity 1, human centrin 1 97 residues - Formula weight is not available
| 1 | MET | ALA | SER | GLY | PHE | LYS | LYS | PRO | SER | ALA | ||||
| 2 | ALA | SER | THR | GLY | GLN | LYS | ARG | LYS | VAL | ALA | ||||
| 3 | PRO | LYS | PRO | GLU | LEU | THR | GLU | ASP | GLN | LYS | ||||
| 4 | GLN | GLU | VAL | ARG | GLU | ALA | PHE | ASP | LEU | PHE | ||||
| 5 | ASP | VAL | ASP | GLY | SER | GLY | THR | ILE | ASP | ALA | ||||
| 6 | LYS | GLU | LEU | LYS | VAL | ALA | MET | ARG | ALA | LEU | ||||
| 7 | GLY | PHE | GLU | PRO | ARG | LYS | GLU | GLU | MET | LYS | ||||
| 8 | LYS | MET | ILE | SER | GLU | VAL | ASP | ARG | GLU | GLY | ||||
| 9 | THR | GLY | LYS | ILE | SER | PHE | ASN | ASP | PHE | LEU | ||||
| 10 | ALA | VAL | MET | THR | GLN | LYS | MET |
Samples:
sample_1: N-HsCen1, [U-15N], 1.0 – 1.3 mM
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Cosy DQF | sample_1 | not available | conditions_1 |
| TOCSY | sample_1 | not available | conditions_1 |
| NOESY | sample_1 | not available | conditions_1 |
| 2D (15N-1H) HSQC | sample_1 | not available | conditions_1 |
| 3D NOESy-HSQC | sample_1 | not available | conditions_1 |
| TOCSY-HSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks