BMRB Entry 6687

Name: Solution structure of the N-terminal domain (M1-S98) of human centrin 2
Published: 2006-04-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6687

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal domain (M1-S98) of human centrin 2

Deposition date:

2005-06-13

Original release date:

2006-04-17

Authors:

Yang, A.; Miron, S.; Duchambon, P.; Assairi, L.; Blouquit, Y.; Craescu, C.

Citation:

Citation: Yang, A.; Miron, S.; Duchambon, P.; Assairi, L.; Blouquit, Y.; Craescu, C.. "The N-terminal domain of human centrin 2 has a closed structure, binds calcium with a very low affinity, and plays a role in the protein self-assembly" Biochemistry 45, 880-889 (2006).
PubMed: 16411764

Assembly members:

Assembly members:
Human centrin 2, polymer, 98 residues, 11111.8 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human centrin 2: MASNFKKANMASSSQRKRMS PKPELTEEQKQEIREAFDLF DADGTGTIDVKELKVAMRAL GFEPKKEEIKKMISEIDKEG TGKMNFGDFLTVMTQKMS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
15N chemical shifts	80
1H chemical shifts	558

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-terminal domain of human centrin 2	1

Entities:

Entity 1, N-terminal domain of human centrin 2 98 residues - 11111.8 Da.

1

MET

ALA

SER

ASN

PHE

LYS

ALA

ASN

MET

2

ALA

SER

GLN

ARG

LYS

ARG

MET

SER

3

PRO

LYS

PRO

GLU

LEU

THR

GLU

GLN

LYS

4

GLN

GLU

ILE

ARG

GLU

ALA

PHE

ASP

LEU

PHE

5

ASP

ALA

ASP

GLY

THR

GLY

THR

ILE

ASP

VAL

6

LYS

GLU

LEU

LYS

VAL

ALA

MET

ARG

ALA

LEU

7

GLY

PHE

GLU

PRO

LYS

GLU

ILE

LYS

8

LYS

MET

ILE

SER

GLU

ILE

ASP

LYS

GLU

GLY

9

THR

GLY

LYS

MET

ASN

PHE

GLY

ASP

PHE

LEU

10

THR

VAL

MET

THR

GLN

LYS

MET

SER

Samples:

sample_1: Human centrin 20.7 – 1.5 mM; Tris buffer 20 mM; NaCl 100 mM; H2O 93%; D2O 7%

sample_2: Human centrin 2, [U-15N], 1 mM; Tris buffer 20 mM; NaCl 100 mM; H2O 93%; D2O 7%

sample_3: Human centrin 2 1 mM; Tris buffer 20 mM; NaCl 100 mM; D2O 100%

sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available
3D 15N NOESY-HSQC	not available	not available	not available
TOCSY-HSQC	not available	not available	not available
2D 15N HSQC	not available	not available	not available
2D DQF-COSY	not available	not available	not available
TOCSY	not available	not available	not available
NOESY	not available	not available	not available

Software:

DISCOVER v2.98 - refinement

NMR spectrometers:

Varian UNITY 500 MHz

PDB	1ZMZ
REF	XP_004871114 XP_004871115 XP_004871116 XP_004871117