BMRB Entry 6686
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6686
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Citation: Bentrop, Detlef; Bertini, Ivano; Cremonini, Mauro; Forsen, Sture; Luchinat, Claudio; Malmendal, Anders. "Solution Structure of the Paramagnetic Complex of the N-Terminal Domain of
Calmodulin with Two Ce3+ Ions by 1H NMR." Biochemistry 36, 11605-11618 (1997).
PubMed: 9305950
Assembly members:
calmodulin n-terminal domain, polymer, 76 residues, Formula weight is not available
Ce2-TR1C, non-polymer, Formula weight is not available
Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
calmodulin n-terminal domain: MADQLTEEQIAEFKEAFSLF
DKDGDGTITTKELGTVMRSL
GQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 422 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin n-terminal domain | 1 |
| 2 | Ce2-TR1C 1 | 2 |
| 3 | Ce2-TR1C 2 | 2 |
Entities:
Entity 1, calmodulin n-terminal domain 76 residues - Formula weight is not available
| 1 | MET | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ||||
| 2 | ALA | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ||||
| 3 | ASP | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | ||||
| 4 | LYS | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | ||||
| 5 | GLY | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ||||
| 6 | ASP | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ||||
| 7 | ASN | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | ||||
| 8 | THR | MET | MET | ALA | ARG | LYS |
Entity 2, Ce2-TR1C 1 - Formula weight is not available
| 1 | Ce2-TR1C |
Samples:
sample_1: calmodulin n-terminal domain mM
conditions_1: temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
UXNMR, Bruker - NMR data processing
xwinnmr, Bruker - NMR data processing
XEASY, Prof. Kurt Wuthrich - 2D spectra analysis
DYANA, Peter Guntert - structure calculation
CALIBRA, Peter Guntert -
FANTASIA, L. Banci -
NMR spectrometers:
- Bruker DRX 500 500 MHz
- Bruker AC-P 300 300 MHz
Related Database Links:
| BMRB | 18082 18302 4174 6861 6862 |
| PDB | |
| EMBL | CAA29381 CAB65357 CCD82979 |
| GB | AAH53790 ABW89042 ABW89043 ABW89044 ABW89045 |
| REF | XP_001869424 XP_001995129 XP_002772218 XP_005112967 XP_005391108 |