BMRB Entry 6680
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6680
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Citation: He, Yanan; Yeh, D.; Alexander, Patrick; Bryan, Philip; Orban, John. "Solution NMR structures of IgG binding domains with artificially evolved high
levels of sequence identity but different folds" Biochemistry 44, 14055-14061 (2005).
PubMed: 16245921
Assembly members:
A219, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
A219: MYYLVVNKQQNAFYEVLNMP
NLNEDQRNAFIQSLKDDPSQ
SANVLAEAQKLNDVQAPKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 181 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 399 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A219 monomer | 1 |
Entities:
Entity 1, A219 monomer 59 residues - Formula weight is not available
| 1 | MET | TYR | TYR | LEU | VAL | VAL | ASN | LYS | GLN | GLN | ||||
| 2 | ASN | ALA | PHE | TYR | GLU | VAL | LEU | ASN | MET | PRO | ||||
| 3 | ASN | LEU | ASN | GLU | ASP | GLN | ARG | ASN | ALA | PHE | ||||
| 4 | ILE | GLN | SER | LEU | LYS | ASP | ASP | PRO | SER | GLN | ||||
| 5 | SER | ALA | ASN | VAL | LEU | ALA | GLU | ALA | GLN | LYS | ||||
| 6 | LEU | ASN | ASP | VAL | GLN | ALA | PRO | LYS | ALA |
Samples:
A219_1: A219, [U-13C; U-15N], 0.2 – 0.4 mM; ammonium bicarbonate 2 mM; postassium phosphate buffer 100 mM; D2O 10%
A219_2: A2190.2 – 0.4 mM; ammonium bicarbonate 2 mM; postassium phosphate buffer 100 mM; D2O 10%
conditions_1: pH: 7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D CBCACONH | not available | not available | not available |
| 3D HBHA(CBCACO)NH | not available | not available | not available |
| 3D(H)C(CO)NH-TOCSY | not available | not available | not available |
| 3DH(CCO)NH-TOCSY | not available | not available | not available |
| 2DTOCSY | not available | not available | not available |
| 2D-CBHD | not available | not available | not available |
| 2DCBHE | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
Software:
xwinnmr v2.5, Bruker -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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