BMRB Entry 6679
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6679
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: He, Yanan; Yeh, D.; Alexander, Patrick; Bryan, Philip; Orban, John. "Solution NMR structures of IgG binding domains with artificially evolved high
levels of sequence identity but different folds" Biochemistry 44, 14055-14061 (2005).
PubMed: 16245921
Assembly members:
G311, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
G311: MYYLVVNKGQNAFYETLTKA
VDAETARNAFIQSLKDDGVQ
GVWTYDDATKTFTVQA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 226 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 355 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | G311 | 1 |
Entities:
Entity 1, G311 56 residues - Formula weight is not available
| 1 | MET | TYR | TYR | LEU | VAL | VAL | ASN | LYS | GLY | GLN | ||||
| 2 | ASN | ALA | PHE | TYR | GLU | THR | LEU | THR | LYS | ALA | ||||
| 3 | VAL | ASP | ALA | GLU | THR | ALA | ARG | ASN | ALA | PHE | ||||
| 4 | ILE | GLN | SER | LEU | LYS | ASP | ASP | GLY | VAL | GLN | ||||
| 5 | GLY | VAL | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
| 6 | THR | PHE | THR | VAL | GLN | ALA |
Samples:
sample_1: G311, [U-13C; U-15N], 0.2 – 0.4 mM; postassium phosphate buffer 100 mM; D2O 10%
sample_2: G311, [U-15N], 0.2 – 0.4 mM; postassium phosphate buffer 100 mM; D2O 10%
conditions_1: pH: 7; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | not available | not available | conditions_1 |
| 3D HNCO | not available | not available | conditions_1 |
| 3D CBCACONH | not available | not available | conditions_1 |
| 3D HBHA(CBCACO)NH | not available | not available | conditions_1 |
| 3D(H)C(CO)NH-TOCSY | not available | not available | conditions_1 |
| 3d h(CCO)NH-TOCSY | not available | not available | conditions_1 |
| 2D TOCSY | not available | not available | conditions_1 |
| 2D CBHD | not available | not available | conditions_1 |
| 2D CBHE | not available | not available | conditions_1 |
| 2D TOCSY | not available | not available | conditions_1 |
Software:
xwinnmr v2.5, Bruker -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks