BMRB Entry 6661
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6661
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Citation: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John. "Changes in hydrogen-bond strengths explain reduction potentials in 10
rubredoxin variants." Proc. Natl. Acad. Sci. U. S. A. 11, 14581-14586 (2005).
Assembly members:
rubredoxin, polymer, 54 residues, Formula weight is not available
FE, non-polymer, 55.845 Da.
Natural source: Common Name: Clostridium pasteurianum Taxonomy ID: 1501 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium pasteurianum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
rubredoxin: MKKYTCTACGYIYNPEDGDP
DNGVNPGTDFKDIPDDWVCP
LCGVGKDQFEEVEE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 12 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rubredoxin (V8A) | 1 |
| 2 | FE (III) ION | 2 |
Entities:
Entity 1, rubredoxin (V8A) 54 residues - Formula weight is not available
| 1 | MET | LYS | LYS | TYR | THR | CYS | THR | ALA | CYS | GLY | ||||
| 2 | TYR | ILE | TYR | ASN | PRO | GLU | ASP | GLY | ASP | PRO | ||||
| 3 | ASP | ASN | GLY | VAL | ASN | PRO | GLY | THR | ASP | PHE | ||||
| 4 | LYS | ASP | ILE | PRO | ASP | ASP | TRP | VAL | CYS | PRO | ||||
| 5 | LEU | CYS | GLY | VAL | GLY | LYS | ASP | GLN | PHE | GLU | ||||
| 6 | GLU | VAL | GLU | GLU |
Entity 2, FE (III) ION - Fe - 55.845 Da.
| 1 | FE |
Samples:
reduced_rubredoxin_(V8A): rubredoxin (V8A), [U-15], 4 – 8 mM; phosphate buffer 50 mM
50mM_phosphate_buffer: pH: 6; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 15N | reduced_rubredoxin_(V8A) | not available | 50mM_phosphate_buffer |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz