BMRB Entry 6655

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6655
MolProbity Validation Chart

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Title:
Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double stranded RNA binding protein ZFa
Deposition date:
2005-06-06
Original release date:
2005-11-01
Authors:
Wright, Peter; Moller, Heiko; Martinez-Yamout, Maria; Dyson, H.
Citation:

Citation: Moller, Heiko; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double-stranded RNA-binding Protein ZFa."  J. Mol. Biol. 351, 718-730 (2005).
PubMed: 16051273

Assembly members:

Assembly members:
dsRBP-ZFa, polymer, 127 residues, 14048.8 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Xenopus Laevis   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dsRBP-ZFa: ADEFGNGDALDLPVGKDAVN SLIRENSHIFSDTQCKVCSA VLISESQKLAHYQSRKHANK VRRYMAINQGEDSVPAKKFK AAPAEISDGEDRSKCCPVCN MTFSSPVVAESHYIGKTHIK NLRLREQ

Data sets:
Data typeCount
13C chemical shifts528
15N chemical shifts141
1H chemical shifts825

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1dsRBP-ZFa1
2Zinc ion, I2
3Zinc ion, II2

Entities:

Entity 1, dsRBP-ZFa 127 residues - 14048.8 Da.

1   ALAASPGLUPHEGLYASNGLYASPALALEU
2   ASPLEUPROVALGLYLYSASPALAVALASN
3   SERLEUILEARGGLUASNSERHISILEPHE
4   SERASPTHRGLNCYSLYSVALCYSSERALA
5   VALLEUILESERGLUSERGLNLYSLEUALA
6   HISTYRGLNSERARGLYSHISALAASNLYS
7   VALARGARGTYRMETALAILEASNGLNGLY
8   GLUASPSERVALPROALALYSLYSPHELYS
9   ALAALAPROALAGLUILESERASPGLYGLU
10   ASPARGSERLYSCYSCYSPROVALCYSASN
11   METTHRPHESERSERPROVALVALALAGLU
12   SERHISTYRILEGLYLYSTHRHISILELYS
13   ASNLEUARGLEUARGGLUGLN

Entity 2, Zinc ion, I - Zn - 65.409 Da.

1   ZN

Samples:

15N-dsRBP-ZFa: dsRBP-ZFa, [U-98% 15N], 1 ± 0.2 mM; Zinc ion 2 ± 0.4 mM; H2O 95%; D2O 5%; NaCl 100 mM; Tris 25 mM; DTT 2 mM

15N-13C-H2O-dsRBP-ZFa: dsRBP-ZFa, [U-98% 15N; U-98% 13C], 1 ± 0.2 mM; Zinc ion 2 ± 0.4 mM; H2O 95%; D2O 5%; NaCl 100 mM; Tris 25 mM; DTT 2 mM

15N-13C-D2O-dsRBP-ZFa: dsRBP-ZFa, [U-98% 15N; U-98% 13C], 1 ± 0.2 mM; Zinc ion 2 ± 0.4 mM; D2O 100%; NaCl 100 mM; Tris 25 mM; DTT 2 mM

conditions_1: ionic strength: 0.1 M; pH: 6.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC15N-dsRBP-ZFanot availableconditions_1
3D_1H15N_TOCSY_HSQC15N-dsRBP-ZFanot availableconditions_1
3D_1H15N_NOESY_HSQC15N-dsRBP-ZFanot availableconditions_1
1H15N_2J_HMQC15N-dsRBP-ZFanot availableconditions_1
3D_HNHA15N-dsRBP-ZFanot availableconditions_1
1H13C_HSQC15N-13C-D2O-dsRBP-ZFanot availableconditions_1
3D_HNCACB15N-13C-H2O-dsRBP-ZFanot availableconditions_1
3D_CBCACONH15N-13C-H2O-dsRBP-ZFanot availableconditions_1
3D_HNCO15N-13C-H2O-dsRBP-ZFanot availableconditions_1
3D_HCCH_COSY15N-13C-D2O-dsRBP-ZFanot availableconditions_1
3D_HCCH_TOCSY15N-13C-D2O-dsRBP-ZFanot availableconditions_1
3D_1H13C_NOESY_HSQC15N-13C-D2O-dsRBP-ZFanot availableconditions_1
3D_HACAHB_COSY15N-13C-D2O-dsRBP-ZFanot availableconditions_1
13C13CO_SED_CT_HSQC15N-13C-D2O-dsRBP-ZFanot availableconditions_1
13C15N_SED_CT_HSQC15N-13C-D2O-dsRBP-ZFanot availableconditions_1
3D_HBHACBCACONH15N-13C-H2O-dsRBP-ZFanot availableconditions_1

Software:

NMRPipe v2.3, NIH - processing

xwinnmr v3.5, Bruker - acquisition

NMRView v5.0.4, One moon scientific - analysis

CYANA v1.0.5, L.A. Systems - analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks