BMRB Entry 6650

Name: 1H Chemical Shift Assignments for fragments of Sticholysin protein
Published: 2007-09-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6650

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H Chemical Shift Assignments for fragments of Sticholysin protein

Deposition date:

2005-05-31

Original release date:

2007-09-26

Authors:

Oliveira, Aline; Cilli, Eduardo; Alvarez, Carlos; Spisni, Alberto; Pertinhez, Thelma

Citation:

Citation: Casallanovo, Fabio; Oliveira, Felipe; Souza, Fernando; Ros, Uris; Martinez, Yohanka; Penton, David; Tejuca, Mayra; Martinez, Diana; Pazos, Fabiola; Pertinhez, Thelma; Spisni, Alberto; Cilli, Eduardo; Lanio, Maria; Alvarez, Carlos; Schreier, Shirley. "Model peptides mimic the structure and function of the N-terminus of the pore-forming toxin sticholysin II" Biopolymers 84, 169-180 (2006).
PubMed: 16170802

Assembly members:

Assembly members:
St II (1-30) - synthetic fragment encompassing residues 1 to 30 of Sticholysin protein, polymer, 30 residues, 3128.7 Da.

Natural source:

Natural source: Common Name: Stichodactyla helianthus Taxonomy ID: 6123 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Stichodactyla helianthus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
St II (1-30) - synthetic fragment encompassing residues 1 to 30 of Sticholysin protein: ALAGTIIAGASLTFQVLDKV LEELGKVSRK

Data sets:

assigned_chemical_shifts
- st2(1-30)_list

Data type	Count
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	St II fragments	1

Entities:

Entity 1, St II fragments 30 residues - 3128.7 Da.

1	ALA	LEU	ALA	GLY	THR	ILE	ILE	ALA	GLY	ALA
2	SER	LEU	THR	PHE	GLN	VAL	LEU	ASP	LYS	VAL
3	LEU	GLU	GLU	LEU	GLY	LYS	VAL	SER	ARG	LYS

Samples:

sample_1: St II (1-30) 1 mM; SDS 100 mM

SDS: pH: 4.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-TOCSY	sample_1	not available	SDS
1H-NOESY	sample_1	not available	SDS
1H-GMQCOSY	sample_1	not available	SDS

Software:

NMRView v5.0 -

NMRPipe -

DYANA v1.5 -

InsightII v2000, Accelrys Inc. -

ProcheckNMR v3.4 -

DISCOVER v2.98 -

NMR spectrometers:

Varian INOVA 500 MHz

BMRB	16630
PDB	1GWY 1O71 1O72 2L2B
EMBL	CAC20912
SP	P07845
AlphaFold	P07845

1	ALA	LEU	ALA	GLY	THR	ILE	ILE	ALA	GLY	ALA
2	SER	LEU	THR	PHE	GLN	VAL	LEU	ASP	LYS	VAL
3	LEU	GLU	GLU	LEU	GLY	LYS	VAL	SER	ARG	LYS

1	ALA	LEU	ALA	GLY	THR	ILE	ILE	ALA	GLY	ALA
2	SER	LEU	THR	PHE	GLN	VAL	LEU	ASP	LYS	VAL
3	LEU	GLU	GLU	LEU	GLY	LYS	VAL	SER	ARG	LYS