BMRB Entry 6633

Name: Structure of the RNA signal essential for translational frameshifting in HIV-1
Published: 2005-08-18

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PDB ID: 1zc5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6633
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the RNA signal essential for translational frameshifting in HIV-1

Deposition date:

2005-05-13

Original release date:

2005-08-18

Authors:

Gaudin, C.; Mazauric, M.; Traikia, M.; Guittet, E.; Yoshizawa, S.; Fourmy, D.

Citation:

Citation: Gaudin, C.; Mazauric, M.; Traikia, M.; Guittet, E.; Yoshizawa, S.; Fourmy, D.. "Structure of the RNA Signal Essential for Translational Frameshifting in HIV-1" J. Mol. Biol. 349, 1024-1035 (2005).
PubMed: 15907937

Assembly members:

Assembly members:
HIV frameshift RNA signal, polymer, 41 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Lentivirus Taxonomy ID: 11646 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HIV frameshift RNA signal: GGCGAUCUGGCCUUCCUACA AGGGAAGGCCAGGGAAUUGC C

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	18
1H chemical shifts	351

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HIV-1 frameshift RNA signal	1

Entities:

Entity 1, HIV-1 frameshift RNA signal 41 residues - Formula weight is not available

1

G

C

G

A

U

C

U

G

2

C

U

C

U

A

C

A

3

A

G

A

G

C

4

A

G

A

U

G

C

5

C

Samples:

sample_1: HIV frameshift RNA signal 1 mM; Na phosphate buffer 10 mM; D2O 10%; H2O 90%

sample_2: HIV frameshift RNA signal 1 mM; Na phosphate buffer 10 mM; D2O 100%

sample_3: HIV frameshift RNA signal, [U-13C; U-15N], 1 mM; Na phosphate buffer 10 mM; D2O 100%

sample_cond_1: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 283 K

sample_cond_2: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available
HP COSY	not available	not available	not available
3D-HCCH-TOCSY	not available	not available	not available
HSQC	not available	not available	not available
3D HCP	not available	not available	not available

Software:

xwinnmr v3.1 - processing

GIFA v4.4 - data analysis

AURELIA - data analysis

SPARKY v3.110 - data analysis

DISCOVER - refinement

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz
Varian INOVA 500 MHz