BMRB Entry 6631

Name: Solution structure of a human ubiquitin-like domain in SF3A1
Published: 2007-11-13

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PDB ID: 1zkh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6631
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a human ubiquitin-like domain in SF3A1

Deposition date:

2005-05-12

Original release date:

2007-11-13

Authors:

Lukin, J.; Dhe-Paganon, S.; Guido, V.; Arrowsmith, C.

Citation:

Citation: Avvakumov, G.; Xue, S.; Newman, E.; Mackenzie, F.; Dhe-Paganon, S.. "Solution structure of a human ubiquitin-like domain in SF3A1" .

Assembly members:

Assembly members:
Splicing factor 3, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Splicing factor 3: PVSIKVQVPNMQDKTEWKLN GQVLVFTLPLTDQVSVIKVK IHEATGMPAGKQKLQYEGIF IKDSNSLAYYNMANGAVIHL ALKERG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	587
13C chemical shifts	365
15N chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Splicing factor 3 subunit 1	1

Entities:

Entity 1, Splicing factor 3 subunit 1 86 residues - Formula weight is not available

1

PRO

VAL

SER

ILE

LYS

VAL

GLN

VAL

PRO

ASN

2

MET

GLN

ASP

LYS

THR

GLU

TRP

LYS

LEU

ASN

3

GLY

GLN

VAL

LEU

VAL

PHE

THR

LEU

PRO

LEU

4

THR

ASP

GLN

VAL

SER

VAL

ILE

LYS

VAL

LYS

5

ILE

HIS

GLU

ALA

THR

GLY

MET

PRO

ALA

GLY

6

LYS

GLN

LYS

LEU

GLN

TYR

GLU

GLY

ILE

PHE

7

ILE

LYS

ASP

SER

ASN

SER

LEU

ALA

TYR

8

ASN

MET

ALA

ASN

GLY

ALA

VAL

ILE

HIS

LEU

9

ALA

LEU

LYS

GLU

ARG

GLY

Samples:

sample_1: Splicing factor 3, [U-15N; U-13C], 2 mM; NaCl 300 mM; benzamidine 1 mM; DTT 10 mM; NaN3 0.01%; Sodium Acetate 10 mM; ZnSO4 0.01 mM; H2O 90%; D2O 10%

sample_2: Splicing factor 3, [U-15N; U-13C], 2 mM; NaCl 300 mM; benzamidine 1 mM; DTT 10 mM; NaN3 0.01%; Sodium Acetate 10 mM; ZnSO4 0.01 mM; D2O 99.9%

sample_cond_1: pH: 5.0; temperature: 298 K; ionic strength: 300 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

XWINNMR v3.6 - collection

NMRPipe v2.1 - processing

CYANA v2.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

Bruker AVANCE 600 MHz

PDB	1WE7 1ZKH
DBJ	BAC26142 BAC26294 BAG64353 BAG72797 BAK62823
EMBL	CAA59494 CAG30461 CAK54605 CAK54904
GB	AAC23435 AAH01976 AAH07684 AAH10727 AAH29753
REF	NP_001074979 NP_001100705 NP_001244628 NP_001267465 NP_005868
SP	A2VDN6 Q15459 Q8K4Z5
TPG	DAA20517
AlphaFold	A2VDN6 Q15459 Q8K4Z5