BMRB Entry 6628

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6628

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Title:
Specificity and Mechanism of the Histone Methyltransferase Pr-Set7
Deposition date:
2005-05-12
Original release date:
2005-11-22
Authors:
Xiao, Bing; Jing, Chun; Kelly, Geoff; Walker, Philip; Muskett, Frederick; Frenkiel, Thomas; Martin, Steve; Sarma, Kavitha; Reinberg, Danny; Gamblin, Steven; Wilson, Jonathan
Citation:

Citation: Xiao, Bing; Jing, Chun; Kelly, Geoff; Walker, Philip; Muskett, Frederick; Frenkiel, Thomas; Martin, Steve; Sarma, Kavitha; Reinberg, Danny; Gamblin, Steven; Wilson, Jonathan. "Specificity and mechanism of the histone methyltransferase Pr-Set7"  Genes Dev. 19, 1444-1454 (2005).
PubMed: 15933069

Assembly members:

Assembly members:
set7/9, polymer, 264 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
set7/9: GPLGSGQYKDNIRHGVCWIY YPDGGSLVGEVNEDGEMTGE KIAYVYPDERTALYGKFIDG EMIEGKLATLMSTEEGRPHF ELMPGNSVYHFDKSTSSCIS TNALLPDPYESERVYVAESL ISSAGEGLFSKVAVGPNTVM SFYNGVRITHQEVDSRDWAL NGNTLSLDEETVIDVPEPYN HVSKYCASLGHKANHSFTPN CIYDMFVHPRFGPIKCIRTL RAVEADEELTVAYGYDHSPP GKSGPEAPEWYQVELKAFQA TQQK

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts179
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Set7/91

Entities:

Entity 1, Set7/9 264 residues - Formula weight is not available

1   GLYPROLEUGLYSERGLYGLNTYRLYSASP
2   ASNILEARGHISGLYVALCYSTRPILETYR
3   TYRPROASPGLYGLYSERLEUVALGLYGLU
4   VALASNGLUASPGLYGLUMETTHRGLYGLU
5   LYSILEALATYRVALTYRPROASPGLUARG
6   THRALALEUTYRGLYLYSPHEILEASPGLY
7   GLUMETILEGLUGLYLYSLEUALATHRLEU
8   METSERTHRGLUGLUGLYARGPROHISPHE
9   GLULEUMETPROGLYASNSERVALTYRHIS
10   PHEASPLYSSERTHRSERSERCYSILESER
11   THRASNALALEULEUPROASPPROTYRGLU
12   SERGLUARGVALTYRVALALAGLUSERLEU
13   ILESERSERALAGLYGLUGLYLEUPHESER
14   LYSVALALAVALGLYPROASNTHRVALMET
15   SERPHETYRASNGLYVALARGILETHRHIS
16   GLNGLUVALASPSERARGASPTRPALALEU
17   ASNGLYASNTHRLEUSERLEUASPGLUGLU
18   THRVALILEASPVALPROGLUPROTYRASN
19   HISVALSERLYSTYRCYSALASERLEUGLY
20   HISLYSALAASNHISSERPHETHRPROASN
21   CYSILETYRASPMETPHEVALHISPROARG
22   PHEGLYPROILELYSCYSILEARGTHRLEU
23   ARGALAVALGLUALAASPGLUGLULEUTHR
24   VALALATYRGLYTYRASPHISSERPROPRO
25   GLYLYSSERGLYPROGLUALAPROGLUTRP
26   TYRGLNVALGLULEULYSALAPHEGLNALA
27   THRGLNGLNLYS

Samples:

sample_1: set7/9, [U-98% 13C; U-99% 15N; U-98% 2H], 0.5 mM; Tris 20 mM; TCEP 0.5 mM

conditions_1: ionic strength: 0.1 M; pH: 7.0

Experiments:

NameSampleSample stateSample conditions
1H{15N}-TROSYsample_1not availableconditions_1
TROSY-HNCAnot availablenot availablenot available
TROSY-HN(CO)CAnot availablenot availablenot available
TROSY-HN(CA)COnot availablenot availablenot available
TROSY-HNCOnot availablenot availablenot available
TROSY-HN(CA)CBnot availablenot availablenot available
TROSY-HN(COCA)CBnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAB21808 BAG11355
GB AAI21056 AAI21057 AAL56579 AAL69901 AAY40937
REF NP_001244432 NP_085151 XP_002745388 XP_002815202 XP_002916185
SP Q8WTS6
TPG DAA20878
AlphaFold Q8WTS6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks