BMRB Entry 6626

Title:
'Complete 1H, 13C, and 15N Chemical Shift Assignments for plant Tom20 mitochondrial import receptor cytosolic domain (isoform 3) from Arabidopsis thaliana'
Deposition date:
2005-05-12
Original release date:
2005-12-14
Authors:
Perry, Andrew; Hulett, Joanne; Lithgow, Trevor; Gooley, Paul
Citation:

Citation: Perry, Andrew; Hulett, Joanne; Lithgow, Trevor; Gooley, Paul. "1H, 13C and 15N Resonance Assignments of the Cytosolic Domain of Tom20 from Arabidopsis thaliana"  J. Biomol. NMR 33, 198-198 (2005).

Assembly members:

Assembly members:
Plant Tom20 receptor, polymer, 158 residues, 17954.8 Da.

Natural source:

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Vector: pGEX-6-3P-AtTom20-3

Experimental source:

Natural source:   Common Name: Mouse-ear cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Vector: pGEX-6-3P-AtTom20-3

Data sets:
Data typeCount
13C chemical shifts620
15N chemical shifts159
1H chemical shifts1039

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Plant Tom201

Entities:

Entity 1, Plant Tom20 158 residues - 17954.8 Da.

1   GLYPROLEUGLYSERMETASPTHRGLUTHR
2   GLUPHEASPARGILELEULEUPHEGLUGLN
3   ILEARGGLNASPALAGLUASNTHRTYRLYS
4   SERASNPROLEUASPALAASPASNLEUTHR
5   ARGTRPGLYGLYVALLEULEUGLULEUSER
6   GLNPHEHISSERILESERASPALALYSGLN
7   METILEGLNGLUALAILETHRLYSPHEGLU
8   GLUALALEULEUILEASPPROLYSLYSASP
9   GLUALAVALTRPCYSILEGLYASNALATYR
10   THRSERPHEALAPHELEUTHRPROASPGLU
11   THRGLUALALYSHISASNPHEASPLEUALA
12   THRGLNPHEPHEGLNGLNALAVALASPGLU
13   GLNPROASPASNTHRHISTYRLEULYSSER
14   LEUGLUMETTHRALALYSALAPROGLNLEU
15   HISALAGLUALATYRLYSGLNGLYLEUGLY
16   GLYSERHISHISHISHISHISHIS

Samples:

sample_1: Plant Tom20 receptor, [U-95% 13C; U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

sample_2: Plant Tom20 receptor, [U-95% 13C; U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

sample_3: Plant Tom20 receptor, [U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableconditions_1
13C_1H-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
C(CO)NH-TOCSYsample_1not availableconditions_1
H(CCO)NH-TOCSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
13C HSQC-NOESYsample_1not availableconditions_1
13C NOESY-HSQC (aromatic region)sample_1not availableconditions_1
[13C,1H]-HSQC (aromatic region)sample_1not availableconditions_1
15N NOESY-HSQCsample_1not availableconditions_1

Software:

NMRPipe v1.1, Frank Delaglio - spectra processing

SPARKY v3.110, T. D. Goddard and D. G. Kneller - spectral assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAB01089
EMBL CAC14430
GB AAK64184 AAL85142 AAM64598 AEE77264
REF NP_189344
SP P82874
AlphaFold P82874

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks