BMRB Entry 6617

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6617

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Title:
Backbone 1H,15N assignment of the C-terminal haemopexin-like domain of matrix metalloproteinase MMP-13 (collagenase-3)
Deposition date:
2005-05-03
Original release date:
2005-10-26
Authors:
Jacobs, Doris; Grimme, Susanne; Elshorst, Bettina; Pescatore, Barbara; Vogtherr, Martin; Saxena, Krishna; Betz, Marco; Schieborr, Ulrich; Langer, Thomas; Schwalbe, Harald; Fiebig, Klaus
Citation:

Citation: Jacobs, Doris; Grimme, Susanne; Elshorst, Bettina; Pescatore, Barbara; Vogtherr, Martin; Betz, Marco; Schieborr, Ulrich; Langer, Thomas; Saxena, Krishna; Schwalbe, Harald; Fiebig, Klaus. "Backbone NMR Assignment of the C-terminal Haemopexin-like Domain (HPLD) of Human Matrix Metalloproteinase MMP-13"  J. Biomol. NMR 32, 337-337 (2005).
PubMed: 16211490

Assembly members:

Assembly members:
c-terminal haemopexin-like domain of matrix metalloproteinase MMP-13, polymer, 207 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
c-terminal haemopexin-like domain of matrix metalloproteinase MMP-13: LYGPGDEDPNPKHPKTPDKC DPSLSLDAITSLRGETMIFK DRFFWRLHPQQVDAELFLTK SFWPELPNRIDAAYEHPSHD LIFIFRGRKFWALNGYDILE GYPKKISELGLPKEVKKISA AVHFEDTGKTLLFSGNQVWR YDDTNHIMDKDYPRLIEEDF PGIGDKVDAVYEKNGYIYFF NGPIQFEYSIWSNRIVRVMP ANSILWC

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts159
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1matrix metalloproteinase MMP-131
2CALCIUM (II) ION, 12
3CALCIUM (II) ION, 22

Entities:

Entity 1, matrix metalloproteinase MMP-13 207 residues - Formula weight is not available

1 LEU correspond to 259 LEU and 207 CYS correspond to 466 CYS in reference citation. The numbering of the reference citation lacks the sequence number 367.

1   LEUTYRGLYPROGLYASPGLUASPPROASN
2   PROLYSHISPROLYSTHRPROASPLYSCYS
3   ASPPROSERLEUSERLEUASPALAILETHR
4   SERLEUARGGLYGLUTHRMETILEPHELYS
5   ASPARGPHEPHETRPARGLEUHISPROGLN
6   GLNVALASPALAGLULEUPHELEUTHRLYS
7   SERPHETRPPROGLULEUPROASNARGILE
8   ASPALAALATYRGLUHISPROSERHISASP
9   LEUILEPHEILEPHEARGGLYARGLYSPHE
10   TRPALALEUASNGLYTYRASPILELEUGLU
11   GLYTYRPROLYSLYSILESERGLULEUGLY
12   LEUPROLYSGLUVALLYSLYSILESERALA
13   ALAVALHISPHEGLUASPTHRGLYLYSTHR
14   LEULEUPHESERGLYASNGLNVALTRPARG
15   TYRASPASPTHRASNHISILEMETASPLYS
16   ASPTYRPROARGLEUILEGLUGLUASPPHE
17   PROGLYILEGLYASPLYSVALASPALAVAL
18   TYRGLULYSASNGLYTYRILETYRPHEPHE
19   ASNGLYPROILEGLNPHEGLUTYRSERILE
20   TRPSERASNARGILEVALARGVALMETPRO
21   ALAASNSERILELEUTRPCYS

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: matrix metalloproteinase MMP-13, [U-13C; U-15N; U-50% 2H], 0.7 mM; BisTris 20 mM; Na2SO4 200 mM; CaCl2 5 mM; Arginine 10 mM

conditions_1: ionic strength: 0.2 M; pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOCACBsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCOsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks