BMRB Entry 6615

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SSD domain of Bacillus subtilis Lon protease
Published: 2005-12-29

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PDB ID: 1x37
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6615
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for SSD domain of Bacillus subtilis Lon protease

Deposition date:

2005-04-29

Original release date:

2005-12-29

Authors:

Wang, Iren; Lou, Yuan-Chao; Lo, Shih-Chi; Lee, Yueh-Luen; Wu, Shih-Hsiung; Chen, Chinpan

Citation:

Citation: Wang, Iren; Lou, Yuan-Chao; Lin, Yu-Ching; Lo, Shih-Chi; Lee, AlanYueh-Luen; Wu, Shih-Hsiung; Chen, Chinpan. "(1)H, (13)C and (15)N resonance assignments of alpha-domain for Bacillus subtilis Lon protease" Biomol. NMR Assignments 1, 201-203 (2007).
PubMed: 19636865

Assembly members:

Assembly members:
BsLonSSD, polymer, 124 residues, 13965 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BsLonSSD: MAGYTEIEKLEIVKDHLLPK QIKEHGLKKSNLQLRDQAIL DIIRYYTREAGVRSLERQLA AICRKAAKAIVAEERKRITV TEKNLQDFIGKRIFRYGQAE TEDQVGVVTGLAYTTVLRHH HHHH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	516
15N chemical shifts	120
1H chemical shifts	845

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BsLonSSD monomer	1

Entities:

Entity 1, BsLonSSD monomer 124 residues - 13965 Da.

1

MET

ALA

GLY

TYR

THR

GLU

ILE

GLU

LYS

LEU

2

GLU

ILE

VAL

LYS

ASP

HIS

LEU

PRO

LYS

3

GLN

ILE

LYS

GLU

HIS

GLY

LEU

LYS

SER

4

ASN

LEU

GLN

LEU

ARG

ASP

GLN

ALA

ILE

LEU

5

ASP

ILE

ARG

TYR

THR

ARG

GLU

ALA

6

GLY

VAL

ARG

SER

LEU

GLU

ARG

GLN

LEU

ALA

7

ALA

ILE

CYS

ARG

LYS

ALA

LYS

ALA

ILE

8

VAL

ALA

GLU

ARG

LYS

ARG

ILE

THR

VAL

9

THR

GLU

LYS

ASN

LEU

GLN

ASP

PHE

ILE

GLY

10

LYS

ARG

ILE

PHE

ARG

TYR

GLY

GLN

ALA

GLU

11

THR

GLU

ASP

GLN

VAL

GLY

VAL

THR

GLY

12

LEU

ALA

TYR

THR

VAL

LEU

ARG

HIS

13

HIS

Samples:

sample_1: BsLonSSD, [U-95% 13C; U-95% 15N], 1.5 ± 0.2 mM; D2O 10%; phosphate buffer 50 mM; sodium chloride 100 mM; Argnine 50 mM; glutamic acid 50 mM; DTT 5 mM

conditions_1: pH: 5.8; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
3D HBHA(CO)NH	sample_1	not available	conditions_1
3D HCCH-TOCSY	sample_1	not available	conditions_1
3D CBCA(CO)NH	sample_1	not available	conditions_1
CCCONH	sample_1	not available	conditions_1
3D 15N-NOESY spectra	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	1X37 3M6A
DBJ	BAI86310 BAM53256 BAM58898 GAK80335
EMBL	CAA53984 CAA99540 CAB14780 CCU59313 CEI58050
GB	ADM38771 ADV93612 AEP87668 AEP91815 AFI29338
REF	NP_390698 WP_003222565 WP_003229618 WP_003237606 WP_014114674
SP	P37945
AlphaFold	P37945