BMRB Entry 6606

Name: backbone and side chain assignment for RPA70A from S.cerevisiae
Published: 2005-10-27

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6606

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

backbone and side chain assignment for RPA70A from S.cerevisiae

Deposition date:

2005-04-22

Original release date:

2005-10-27

Authors:

Park, Chin-Ju; Lee, Joon-Hwa; Choi, Byong-Seok

Citation:

Citation: Park, Chin-Ju; Lee, Joon-Hwa; Choi, Byong-Seok. "NMR Assignment of the DNA Binding Domain A of RPA from S. cerevisiae" J. Biomol. NMR 33, 75-75 (2005).
PubMed: 16222565

Assembly members:

Assembly members:
scRPA70A, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
scRPA70A: TRPIFAIEQLSPYQNVWTIK ARVSYKGEIKTWHNQRGDGK LFNVNFLDTSGEIRATAFND FATKFNEILQEGKVYYVSKA KLQPAKPQFTNLTHPYELNL DRDTVIEECFDESN

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	317
15N chemical shifts	115
1H chemical shifts	746

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA binding domain A of RPA70kDa subunit	1

Entities:

Entity 1, DNA binding domain A of RPA70kDa subunit 114 residues - Formula weight is not available

1

THR

ARG

PRO

ILE

PHE

ALA

ILE

GLU

GLN

LEU

2

SER

PRO

TYR

GLN

ASN

VAL

TRP

THR

ILE

LYS

3

ALA

ARG

VAL

SER

TYR

LYS

GLY

GLU

ILE

LYS

4

THR

TRP

HIS

ASN

GLN

ARG

GLY

ASP

GLY

LYS

5

LEU

PHE

ASN

VAL

ASN

PHE

LEU

ASP

THR

SER

6

GLY

GLU

ILE

ARG

ALA

THR

ALA

PHE

ASN

ASP

7

PHE

ALA

THR

LYS

PHE

ASN

GLU

ILE

LEU

GLN

8

GLU

GLY

LYS

VAL

TYR

VAL

SER

LYS

ALA

9

LYS

LEU

GLN

PRO

ALA

LYS

PRO

GLN

PHE

THR

10

ASN

LEU

THR

HIS

PRO

TYR

GLU

LEU

ASN

LEU

11

ASP

ARG

ASP

THR

VAL

ILE

GLU

CYS

PHE

12

ASP

GLU

SER

ASN

Samples:

sample_1: scRPA70A, [U-13C; U-15N], 1 mM; NaPi 20 mM; NaCl 100 mM; DTT 2 mM

sample_2: scRPA70A, [U-15N], 1 mM; NaPi 20 mM; NaCl 100 mM; DTT 2 mM

conditions_1: pH: 6.5; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_2	not available	conditions_1
3-D CBCA(CO)NH	not available	not available	not available
13C-edited NOESY-HSQC	not available	not available	not available
HCCH-TOCSY-NNH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HNCO	not available	not available	not available
HNCACB	not available	not available	not available
15N-Edited NOESY_HSQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz

PDB	1YNX
DBJ	GAA21426
EMBL	CAA42420 CAY77656
GB	AAA34994 AAB27889 AAC04960 AHY74368 EDN59765
REF	NP_009404 XP_011102371
SP	P22336
TPG	DAA06989
AlphaFold	P22336