BMRB Entry 6601

Name: 1H, 13C, and 15N chemical shift assignments for B. subtilis PrsA PPIase
Published: 2006-04-25

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6601

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for B. subtilis PrsA PPIase

Deposition date:

2005-04-19

Original release date:

2006-04-25

Authors:

Tossavainen, Helena; Permi, Perttu; Purhonen, Susanna; Sarvas, Matti; Kilpelainen, Ilkka; Seppala, Raili

Citation:

Citation: Tossavainen, Helena; Permi, Perttu; Purhonen, Susanna; Sarvas, Matti; Kilpelainen, Ilkka; Seppala, Raili. "NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from Bacillus subtilis" FEBS Lett. 580, 1822-1826 (2006).
PubMed: 16516208

Assembly members:

Assembly members:
PrsA PPIase, polymer, 93 residues, 10579 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PrsA PPIase: GSGKIRASHILVADKKTAEE VEKKLKKGEKFEDLAKEYST DSSASKGGDLGWFAKEGQMD ETFSKAAFKLKTGEVSDPVK TQYGYHIIKKTEE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	417
15N chemical shifts	92
1H chemical shifts	636

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PrsA PPIase	1

Entities:

Entity 1, PrsA PPIase 93 residues - 10579 Da.

1

GLY

SER

GLY

LYS

ILE

ARG

ALA

SER

HIS

ILE

2

LEU

VAL

ALA

ASP

LYS

THR

ALA

GLU

3

VAL

GLU

LYS

LEU

LYS

GLY

GLU

LYS

4

PHE

GLU

ASP

LEU

ALA

LYS

GLU

TYR

SER

THR

5

ASP

SER

ALA

SER

LYS

GLY

ASP

LEU

6

GLY

TRP

PHE

ALA

LYS

GLU

GLY

GLN

MET

ASP

7

GLU

THR

PHE

SER

LYS

ALA

PHE

LYS

LEU

8

LYS

THR

GLY

GLU

VAL

SER

ASP

PRO

VAL

LYS

9

THR

GLN

TYR

GLY

TYR

HIS

ILE

LYS

10

THR

GLU

Samples:

sample_1: PrsA PPIase, [U-13C; U-15N], 0.9 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
iHNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
iHNCACB	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
H(CCO)NH	not available	not available	not available
C(CCO)NH	not available	not available	not available
HCCH-COSY	not available	not available	not available
CBCGCDHD	not available	not available	not available
CBCGCGCEHE	not available	not available	not available

Software:

Vnmr v6.1c - Fourier transformation

Sparky v3.106 - peak assignment

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

PDB	1ZK6
EMBL	CAA40543 CAA74418 CAB12835
REF	NP_388876
SWISS-PROT	P24327