BMRB Entry 6584

Name: Backbone assignment of the E2 ubiquitin conjugating enzyme UbcH5alpha
Published: 2005-10-25

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6584

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the E2 ubiquitin conjugating enzyme UbcH5alpha

Deposition date:

2005-04-11

Original release date:

2005-10-25

Authors:

Saxena, Krishna; Jacobs, Doris; Vogtherr, Martin; Grimme, Susanne; Elshorst, Bettina; Fiebig, Klaus; Schwalbe, Harald

Citation:

Citation: Saxena, Krishna; Jacobs, Doris; Vogtherr, Martin; Grimme, Susanne; Elshorst, Bettina; Pescatore, Barbara; Betz, Marco; Schieborr, Ulrich; Langer, Thomas; Schwalbe, Harald; Fiebig, Klaus. "Backbone NMR Assignment of the Human E2 Ubiquitin Conjugating Enzyme UbcH5alpha (F72K,F82S) Double Mutant" J. Biomol. NMR 32, 338-338 (2005).
PubMed: 16211490

Assembly members:

Assembly members:
Ubiquitin ligating enzyme, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin ligating enzyme: MGHHHHHHHHHHSSGHIEGR HMALKRIQKELSDLQRDPPA HCSAGPVGDDLFHWQATIMG PPDSAYQGGVFKLTVHFPTD YPSKPPKIAFTTKIYHPNIN SNGSICLDILRSQWSPALTV SKVLLSICSLLCDPNPDDPL VPDIAQIYKSDKEKYNRHAR EWTQKYAM

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	140
1H chemical shifts	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBCH5alpha	1

Entities:

Entity 1, UBCH5alpha 168 residues - Formula weight is not available

1

MET

GLY

HIS

2

HIS

SER

GLY

HIS

ILE

GLU

GLY

ARG

3

HIS

MET

ALA

LEU

LYS

ARG

ILE

GLN

LYS

GLU

4

LEU

SER

ASP

LEU

GLN

ARG

ASP

PRO

ALA

5

HIS

CYS

SER

ALA

GLY

PRO

VAL

GLY

ASP

6

LEU

PHE

HIS

TRP

GLN

ALA

THR

ILE

MET

GLY

7

PRO

ASP

SER

ALA

TYR

GLN

GLY

VAL

8

PHE

LYS

LEU

THR

VAL

HIS

PHE

PRO

THR

ASP

9

TYR

PRO

SER

LYS

PRO

LYS

ILE

ALA

PHE

10

THR

LYS

ILE

TYR

HIS

PRO

ASN

ILE

ASN

11

SER

ASN

GLY

SER

ILE

CYS

LEU

ASP

ILE

LEU

12

ARG

SER

GLN

TRP

SER

PRO

ALA

LEU

THR

VAL

13

SER

LYS

VAL

LEU

SER

ILE

CYS

SER

LEU

14

LEU

CYS

ASP

PRO

ASN

PRO

ASP

PRO

LEU

15

VAL

PRO

ASP

ILE

ALA

GLN

ILE

TYR

LYS

SER

16

ASP

LYS

GLU

LYS

TYR

ASN

ARG

HIS

ALA

ARG

17

GLU

TRP

THR

GLN

LYS

TYR

ALA

MET

Samples:

sample_1: Ubiquitin ligating enzyme, [U-13C; U-15N; U-60% 2H], 1.7 mM; sodium sulphate 200 mM; sodium phosphate 50 mM; DTT 5 mM

Ex-cond_1: ionic strength: 900 mM; pH: 6.0; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HNCACB	not available	not available	not available
HN(CO)CACB	not available	not available	not available

Software:

XEASY - assignment

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

PDB	2YHO 3OJ4 3PTF 4AP4
DBJ	BAE25905 BAE28506 BAF84590 BAJ20325
EMBL	CAA55019 CAC82097 CAC82177
GB	AAH05980 AAH15997 AAH19464 AAI11176 AAM81086
REF	NP_001033256 NP_001072142 NP_001102000 NP_001186455 NP_001191809
SP	D3ZDK2 P51668 P61080 Q2TA10
TPG	DAA15007
AlphaFold	D3ZDK2 P51668 P61080 Q2TA10