BMRB Entry 6578

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6578

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Bcl-xl/Bad BH3 peptide complex
Deposition date:
2005-04-01
Original release date:
2005-09-01
Authors:
Petros, Andrew; Fesik, Stephen; Olejniczak, Edward
Citation:

Citation: Petros, Andrew; Fesik, Stephen; Olejniczak, Edward. "1H, 13C and 15N Resonance Assignments of a Bcl-xL/Bad Peptide Complex"  J. Biomol. NMR 32, 260-260 (2005).
PubMed: 16132832

Assembly members:

Assembly members:
bcl-xl, polymer, 171 residues, Formula weight is not available
Bad-BH3, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bcl-xl: MSQSNRELVVDFLSYKLSQK GYSWSQFSDVEENRTEAPEG TESEAVKQALREAGDEFELR YRRAFSDLTSQLHITPGTAY QSFEQVVNELFRDGVNWGRI VAFFSFGGALCVESVDKKMQ VLVSRIAAWMATYLNDHLEP WIQENGGWDTFVELYGNNAA AESRKGQERLE
Bad-BH3: NLWAAQRYGRELRRMSDEFV DSFKK

Data sets:
Data typeCount
13C chemical shifts632
15N chemical shifts162
1H chemical shifts983

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bcl-xl1
2Bad-BH32

Entities:

Entity 1, bcl-xl 171 residues - Formula weight is not available

1   METSERGLNSERASNARGGLULEUVALVAL
2   ASPPHELEUSERTYRLYSLEUSERGLNLYS
3   GLYTYRSERTRPSERGLNPHESERASPVAL
4   GLUGLUASNARGTHRGLUALAPROGLUGLY
5   THRGLUSERGLUALAVALLYSGLNALALEU
6   ARGGLUALAGLYASPGLUPHEGLULEUARG
7   TYRARGARGALAPHESERASPLEUTHRSER
8   GLNLEUHISILETHRPROGLYTHRALATYR
9   GLNSERPHEGLUGLNVALVALASNGLULEU
10   PHEARGASPGLYVALASNTRPGLYARGILE
11   VALALAPHEPHESERPHEGLYGLYALALEU
12   CYSVALGLUSERVALASPLYSLYSMETGLN
13   VALLEUVALSERARGILEALAALATRPMET
14   ALATHRTYRLEUASNASPHISLEUGLUPRO
15   TRPILEGLNGLUASNGLYGLYTRPASPTHR
16   PHEVALGLULEUTYRGLYASNASNALAALA
17   ALAGLUSERARGLYSGLYGLNGLUARGLEU
18   GLU

Entity 2, Bad-BH3 25 residues - Formula weight is not available

1   ASNLEUTRPALAALAGLNARGTYRGLYARG
2   GLULEUARGARGMETSERASPGLUPHEVAL
3   ASPSERPHELYSLYS

Samples:

sample_1: bcl-xl, [U-13C; U-15N], 0.5 – 1.0 mM; Bad-BH30.5 – 1.0 mM; sodium phosphate 40 mM

conditions_1: pH: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HNCOCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HCCH Tocsysample_1not availableconditions_1
13C seperated Noesysample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAE72969 BAF84552 BAG64171 BAG70068 BAG70192
EMBL CAG46733 CAG46757
GB AAB36516 AAB72092 AAB88124 AAH01901 AAH95431
REF NP_001238857 NP_001252885 NP_004313 NP_116784 XP_002755519
SP Q92934
AlphaFold Q92934

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks