BMRB Entry 6571

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6571

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Title:
Chemical Shift Assignments for the theta subunit of DNA polymerase III from E. coli
Deposition date:
2005-03-29
Original release date:
2005-10-17
Authors:
Mueller, Geoffrey; Kirby, Thomas; DeRose, Eugene; Li, Dawei; Schaaper, Roel; London, Robert
Citation:

Citation: Mueller, Geoffrey; Kirby, Thomas; DeRose, Eugene; Li, Dawei; Schaaper, Roel; London, Robert. "Nuclear Magnetic Resonance Solution Structure of the Escherichia coli DNA Polymerase III {theta} Subunit."  J. Bacteriol. 187, 7081-7089 (2005).
PubMed: 16199579

Assembly members:

Assembly members:
theta, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
theta: MLKNLAKLDQTEMDKVNVDL AAAGVAFKERYNMPVIAEAV EREQPEHLRSWFRERLIAHR LASVNLSRLPYEPKLK

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts69
1H chemical shifts397

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1.1

Entities:

Entity 1, . 76 residues - Formula weight is not available

1   METLEULYSASNLEUALALYSLEUASPGLN
2   THRGLUMETASPLYSVALASNVALASPLEU
3   ALAALAALAGLYVALALAPHELYSGLUARG
4   TYRASNMETPROVALILEALAGLUALAVAL
5   GLUARGGLUGLNPROGLUHISLEUARGSER
6   TRPPHEARGGLUARGLEUILEALAHISARG
7   LEUALASERVALASNLEUSERARGLEUPRO
8   TYRGLUPROLYSLEULYS

Samples:

sample_1: theta 600 uM; d3-methanol 40 v/v; H20 100%

sample_2: theta 600 uM; d3-methanol 40 v/v; D20 100%

conditions_1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC (example)not availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HCCH-tocsynot availablenot availableconditions_1
3D 15N-edited NOESYnot availablenot availableconditions_1
3d 13C-edited NOESYnot availablenot availableconditions_1
(HB)CB(CGCD)HDnot availablenot availableconditions_1
(HB)CB(CGCDCE)HEnot availablenot availableconditions_1
1H-13C HSQCnot availablenot availableconditions_1
H(CCO)NH-TOCSYnot availablenot availableconditions_1
(H)C(CO)NH-TOCSYnot availablenot availableconditions_1

Software:

nmrPipe and nmrDraw - Processing time domain data

NMRView - analyzing frequency domain data

PACES - automated main chain assignments

NvAssign - Analyzing Main Chain assignment data

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA15650 BAB35975 BAG77541 BAI25919 BAI36214
EMBL CAP76332 CAQ32318 CAQ98768 CAR03202 CAR08239
GB AAA23697 AAA23982 AAC74912 AAG56832 AAN43412
REF NP_288279 NP_310579 NP_416356 NP_707705 NP_754146
SP P0ABS8 P0ABS9 P0ABT0 P0ABT1
AlphaFold P0ABS8 P0ABS9 P0ABT0 P0ABT1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks