BMRB Entry 6555

Name: Solution structure of YBL071w-A from Saccharomyces cerevisiae
Published: 2008-07-01

Click here to enlarge.

PDB ID: 1yws
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6555
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of YBL071w-A from Saccharomyces cerevisiae

Deposition date:

2005-03-18

Original release date:

2008-07-01

Authors:

Lukin, J.; Guido, V.; Arrowsmith, C.

Citation:

Citation: Proudfoot, Michael; Sanders, Stephen; Singer, Alex; Zhang, Rongguang; Brown, Greg; Binkowski, Andrew; Xu, Linda; Lukin, Jonathan; Murzin, Alexey; Joachimiak, Andrzej; Arrowsmith, Cheryl; Edwards, Aled; Savchenko, Alexei; Yakunin, Alexander. "Biochemical and Structural Characterization of a Novel Family of Cystathionine beta-Synthase Domain Proteins Fused to a Zn Ribbon-Like Domain" J. Mol. Biol. 375, 301-315 (2008).
PubMed: 18021800

Assembly members:

Assembly members:
YBL071w-A, polymer, 82 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YBL071w-A: MSTYDEIEIEDMTFEPENQM FTYPCPCGDRFQIYLDDMFE GEKVAVCPSCSLMIDVVFDK EDLAEYYEEAGIHPPEPIAA AA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	451
13C chemical shifts	308
15N chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein YBL071w-A	1
2	ZINC ION	2

Entities:

Entity 1, protein YBL071w-A 82 residues - Formula weight is not available

1

MET

SER

THR

TYR

ASP

GLU

ILE

GLU

ILE

GLU

2

ASP

MET

THR

PHE

GLU

PRO

GLU

ASN

GLN

MET

3

PHE

THR

TYR

PRO

CYS

PRO

CYS

GLY

ASP

ARG

4

PHE

GLN

ILE

TYR

LEU

ASP

MET

PHE

GLU

5

GLY

GLU

LYS

VAL

ALA

VAL

CYS

PRO

SER

CYS

6

SER

LEU

MET

ILE

ASP

VAL

PHE

ASP

LYS

7

GLU

ASP

LEU

ALA

GLU

TYR

GLU

ALA

8

GLY

ILE

HIS

PRO

GLU

PRO

ILE

ALA

9

ALA

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: YBL071w-A, [U-15N; U-13C], 1 mM; NaCl 450 mM; benzamidine 1 mM; NaN3 0.01%; MOPS 10 mM; ZnSO4 0.01 mM; D2O 10%; H2O 90%

sample_2: YBL071w-A, [U-15N; U-13C], 1 mM; NaCl 450 mM; benzamidine 1 mM; NaN3 0.01%; MOPS 10 mM; ZnSO4 0.01 mM; D2O 99.9%

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 450 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	sample_cond_1
3D 13C-separated NOESY	not available	not available	sample_cond_1

Software:

NMRPipe v2.1 - processing

CYANA v2.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

Bruker DRX 600 MHz

REF	NP_660100
SWISS-PROT	Q3E840
GenBank	EDN64549 EDV12157
PDB	1YOP 1YWS