BMRB Entry 6543

Name: HIV-1 frameshift inducing element RNA
Published: 2006-11-06

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PDB ID: 1z2j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6543
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HIV-1 frameshift inducing element RNA

Deposition date:

2005-03-10

Original release date:

2006-11-06

Authors:

Staple, David; Butcher, Samuel

Citation:

Citation: Staple, David; Butcher, Samuel. "Soultion structure and thermodynamic investigation of the HIV-1 frameshift inducing element" J. Mol. Biol. 349, 1011-1023 (2005).
PubMed: 15927637

Assembly members:

Assembly members:
HIV-1 frameshift induciting element RNA, polymer, 45 residues, Formula weight is not available

Natural source:

Natural source: Common Name: HIV Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: Not applicable Genus/species: HIV HIV

Experimental source:

Experimental source: Production method: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HIV-1 frameshift induciting element RNA: GGGAAGAUCUGGCCUUCCCA CAAGGGAAGGCCAGGGAAUC UUCCC

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
1H chemical shifts	308
13C chemical shifts	91
15N chemical shifts	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HIV-1 frameshift inducing RNA	1

Entities:

Entity 1, HIV-1 frameshift inducing RNA 45 residues - Formula weight is not available

1

G

A

G

A

U

C

U

2

G

C

U

C

A

3

C

A

G

A

G

4

C

A

G

A

U

C

5

U

C

Samples:

sample_1: HIV-1 frameshift induciting element RNA, [U-13C; U-15N]-A,U, 1 mM; NaCl 50 mM; D2O 10%; H2O 90%

sample_2: HIV-1 frameshift induciting element RNA, [U-13C; U-15N]-G,C, 1 mM; NaCl 50 mM; D2O 100%

conditions_1: pH: 6.8; pressure: 1 atm; temperature: 303 K

conditions_2: pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	not available	not available	not available
2D 1H-1H TOCSY	not available	not available	not available
2D 1H-13C HSQC	not available	not available	not available
2D 1H-15N HMQC	not available	not available	not available
2D HNN COSY	not available	not available	not available
3D HCCH TOCSY	not available	not available	not available
3D HCCH COSY	not available	not available	not available
3D NOESY-HMQC	not available	not available	not available
Filter/Edit 2D 1H-1H NOEY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DMX 750 MHz
Varian Inova 800 MHz
Varian Inova 900 MHz