BMRB Entry 6542

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6542

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Title:
Main Chain 1H, 13C, and 15N chemical shift assignments for arginine kinase
Deposition date:
2005-03-09
Original release date:
2005-07-26
Authors:
Davulcu, Omar; Clark, Shawn; Chapman, Michael; Skalicky, Jack
Citation:

Citation: Davulcu, Omar; Clark, Shawn; Chapman, Michael; Skalicky, Jack. "Main chain 1H, 13C, and 15N resonance assignments of the 42-kDa enzyme arginine kinase"  J. Biomol. NMR 32, 178-178 (2005).

Assembly members:

Assembly members:
Arginine kinase, polymer, 357 residues, 42000 Da.

Natural source:

Natural source:   Common Name: Horseshoe crab   Taxonomy ID: 6850   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Limulus polyphemus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Arginine kinase: MVDQATLDKLEAGFKKLQEA SDCKSLLKKHLTKDVFDSIK NKKTGMGATLLDVIQSGVEN LDSGVGIYAPDAESYRTFGP LFDPIIDDYHGGFKLTDKHP PKEWGDINTLVDLDPGGQFI ISTRVRCGRSLQGYPFNPCL TAEQYKEMEEKVSSTLSSME DELKGTYYPLTGMSKATQQQ LIDDHFLFKEGDRFLQTANA CRYWPTGRGIFHNDAKTFLV WVNEEDHLRIISMQKGGDLK TVYKRLVTAVDNIESKLPFS HDDRFGFLTFCPTNLGTTMR ASVHIQLPKLAKDRKVLEDI ASKFNLQVRGTRGEHTESEG GVYDISNKRRLGLTEYQAVR EMQDGILEMIKMEKAAA

Data sets:
Data typeCount
13C chemical shifts662
15N chemical shifts328
1H chemical shifts328

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Arginine_kinsae_polypeptide1

Entities:

Entity 1, Arginine_kinsae_polypeptide 357 residues - 42000 Da.

1   METVALASPGLNALATHRLEUASPLYSLEU
2   GLUALAGLYPHELYSLYSLEUGLNGLUALA
3   SERASPCYSLYSSERLEULEULYSLYSHIS
4   LEUTHRLYSASPVALPHEASPSERILELYS
5   ASNLYSLYSTHRGLYMETGLYALATHRLEU
6   LEUASPVALILEGLNSERGLYVALGLUASN
7   LEUASPSERGLYVALGLYILETYRALAPRO
8   ASPALAGLUSERTYRARGTHRPHEGLYPRO
9   LEUPHEASPPROILEILEASPASPTYRHIS
10   GLYGLYPHELYSLEUTHRASPLYSHISPRO
11   PROLYSGLUTRPGLYASPILEASNTHRLEU
12   VALASPLEUASPPROGLYGLYGLNPHEILE
13   ILESERTHRARGVALARGCYSGLYARGSER
14   LEUGLNGLYTYRPROPHEASNPROCYSLEU
15   THRALAGLUGLNTYRLYSGLUMETGLUGLU
16   LYSVALSERSERTHRLEUSERSERMETGLU
17   ASPGLULEULYSGLYTHRTYRTYRPROLEU
18   THRGLYMETSERLYSALATHRGLNGLNGLN
19   LEUILEASPASPHISPHELEUPHELYSGLU
20   GLYASPARGPHELEUGLNTHRALAASNALA
21   CYSARGTYRTRPPROTHRGLYARGGLYILE
22   PHEHISASNASPALALYSTHRPHELEUVAL
23   TRPVALASNGLUGLUASPHISLEUARGILE
24   ILESERMETGLNLYSGLYGLYASPLEULYS
25   THRVALTYRLYSARGLEUVALTHRALAVAL
26   ASPASNILEGLUSERLYSLEUPROPHESER
27   HISASPASPARGPHEGLYPHELEUTHRPHE
28   CYSPROTHRASNLEUGLYTHRTHRMETARG
29   ALASERVALHISILEGLNLEUPROLYSLEU
30   ALALYSASPARGLYSVALLEUGLUASPILE
31   ALASERLYSPHEASNLEUGLNVALARGGLY
32   THRARGGLYGLUHISTHRGLUSERGLUGLY
33   GLYVALTYRASPILESERASNLYSARGARG
34   LEUGLYLEUTHRGLUTYRGLNALAVALARG
35   GLUMETGLNASPGLYILELEUGLUMETILE
36   LYSMETGLULYSALAALAALA

Samples:

sample_1: Arginine kinase, [U-100% 13C; U-100% 15N; U-80% 2H], 1.4 mM; Citric Acid, [U-98% 2,2,4,4 2H], 10 mM; DTT 0.5 mM; Potassium Chloride 50 mM; Sodium Azide 50 uM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_TROSYsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian InovaUNITY 720 MHz MHz

Related Database Links:

BMRB 19208
PDB
GB AAA82169
PRF 2104180A
SP P51541
AlphaFold P51541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks