BMRB Entry 6540

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PDB ID: 2axl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6540
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 13C and 15N resonance assignments of the winged-helix domain of the human Werner syndrome protein

Deposition date:

2005-03-07

Original release date:

2005-08-29

Authors:

Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wang-Yong; Hu, Jin-Shan

Citation:

Citation: Sun, Jian-Zhong; Feng, Han-Qiao; Lin, Guang-Xin; Zeng, Wangyong; Hu, Jin-Shan. "NMR Assignments of the Winged-Helix Domain of Human Werner Syndrome Protein" J. Biomol. NMR 32, 261-261 (2005).

Assembly members:

Assembly members:
The winged-helix domain of WRN, polymer, 144 residues, 16333.65 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
The winged-helix domain of WRN: MDDSEDTSWDFGPQAFKLLS AVDILGEKFGIGLPILFLRG SNSQRLADQYRRHSLFGTGK DQTESWWKAFSRQLITGGFL VEVSRYNKFMKICALTKKGR NWLHKANTESQSLILQANEE LCPKKLLLPSSKTVSSGTKE HCYN

Data sets:

assigned_chemical_shifts
- chemical_shifts

Data type	Count
1H chemical shifts	782
13C chemical shifts	604
15N chemical shifts	149

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	The winged-helix domain of WRN protein	1

Entities:

Entity 1, The winged-helix domain of WRN protein 144 residues - 16333.65 Da.

1

MET

ASP

SER

GLU

ASP

THR

SER

TRP

ASP

2

PHE

GLY

PRO

GLN

ALA

PHE

LYS

LEU

SER

3

ALA

VAL

ASP

ILE

LEU

GLY

GLU

LYS

PHE

GLY

4

ILE

GLY

LEU

PRO

ILE

LEU

PHE

LEU

ARG

GLY

5

SER

ASN

SER

GLN

ARG

LEU

ALA

ASP

GLN

TYR

6

ARG

HIS

SER

LEU

PHE

GLY

THR

GLY

LYS

7

ASP

GLN

THR

GLU

SER

TRP

LYS

ALA

PHE

8

SER

ARG

GLN

LEU

ILE

THR

GLY

PHE

LEU

9

VAL

GLU

VAL

SER

ARG

TYR

ASN

LYS

PHE

MET

10

LYS

ILE

CYS

ALA

LEU

THR

LYS

GLY

ARG

11

ASN

TRP

LEU

HIS

LYS

ALA

ASN

THR

GLU

SER

12

GLN

SER

LEU

ILE

LEU

GLN

ALA

ASN

GLU

13

LEU

CYS

PRO

LYS

LEU

PRO

SER

14

SER

LYS

THR

VAL

SER

GLY

THR

LYS

GLU

15

HIS

CYS

TYR

ASN

Samples:

sample_1: The winged-helix domain of WRN, [U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_2: The winged-helix domain of WRN, [U-98% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_3: The winged-helix domain of WRN, [U-95% 13C; U-95% 15N], 0.45 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

sample_4: The winged-helix domain of WRN 0.29 mM; potassium phosphate buffer 25 mM; NaCl 25 mM; EDTA 1 mM; NaZ3 0.05%

Ex-cond_1: pH: 7.36; temperature: 295 K; ionic strength: 0.025 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
CBCA(CO)NH	not available	not available	Ex-cond_1
HNCO	not available	not available	Ex-cond_1
HNCOCO	not available	not available	Ex-cond_1
HNCG_arom	not available	not available	Ex-cond_1
HN(CO)CG_arom	not available	not available	Ex-cond_1
HNCG	not available	not available	Ex-cond_1
HN(CO)C	not available	not available	Ex-cond_1
HNHA	not available	not available	Ex-cond_1
HBHA(CO)NH	not available	not available	Ex-cond_1
DIPSI-H(CCO)NH	not available	not available	Ex-cond_1
DIPSI-C(CCO)NH	not available	not available	Ex-cond_1
HAHB	not available	not available	Ex-cond_1
HCCH-TOCSY	not available	not available	Ex-cond_1
HCCH-COSY	not available	not available	Ex-cond_1
1H-13C HSQC	not available	not available	Ex-cond_1
15N-HOHAHA-HSQC	not available	not available	Ex-cond_1
15N-NOESY-HSQC	not available	not available	Ex-cond_1
13C-15N NOESY	not available	not available	Ex-cond_1
13C-13C NOESY	not available	not available	Ex-cond_1

Software:

NMRPIPE - data processing, analysis

PIPP - data processing, analysis

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2AXL 3AAF
DBJ	BAD92889 BAJ17933
GB	AAC41981 AAC63361 AAF06162 AAI72840 AAR05448
REF	NP_000544
SP	Q14191
AlphaFold	Q14191