BMRB Entry 6538

Name: 1H, 13C, and 15N Chemical Shift Assignments for 2B4
Published: 2005-05-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6538

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for 2B4

Deposition date:

2005-03-07

Original release date:

2005-05-27

Authors:

Ames, James

Citation:

Citation: Ames, J.; Vyas, V.; Lusin, J.; Mariuzza, R.. "NMR Structure of the Natural Killer Cell Receptor 2B4 (CD244): Implications for Ligand Recognition" Biochemistry. 44, 6416-6423 (2005).

Assembly members:

Assembly members:
D1 domain of 2B4, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D1 domain of 2B4: DCPDSSEEVVGVSGKPVQLR PSNIQTKDVSVQWKKTEQGS HRKIEILNWYNDGPSWSNVS FSDIYGFDYGDFALSIKSAK LQDSGHYLLEITNTGGKVCN KNFQLLILD

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	437
15N chemical shifts	98
1H chemical shifts	641

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	2B4_polypeptide	1

Entities:

Entity 1, 2B4_polypeptide 109 residues - Formula weight is not available

D1 domain of 2B4 starts at residue D21 in the mouse gene sequence. Amino acid sequence was renumbered in this study so that the first residue (D21) now becomes D1.

1

ASP

CYS

PRO

ASP

SER

GLU

VAL

2

GLY

VAL

SER

GLY

LYS

PRO

VAL

GLN

LEU

ARG

3

PRO

SER

ASN

ILE

GLN

THR

LYS

ASP

VAL

SER

4

VAL

GLN

TRP

LYS

THR

GLU

GLN

GLY

SER

5

HIS

ARG

LYS

ILE

GLU

ILE

LEU

ASN

TRP

TYR

6

ASN

ASP

GLY

PRO

SER

TRP

SER

ASN

VAL

SER

7

PHE

SER

ASP

ILE

TYR

GLY

PHE

ASP

TYR

GLY

8

ASP

PHE

ALA

LEU

SER

ILE

LYS

SER

ALA

LYS

9

LEU

GLN

ASP

SER

GLY

HIS

TYR

LEU

GLU

10

ILE

THR

ASN

THR

GLY

LYS

VAL

CYS

ASN

11

LYS

ASN

PHE

GLN

LEU

ILE

LEU

ASP

Samples:

sample_1: D1 domain of 2B4, [U-95% 13C; U-90% 15N], 0.4 mM; sodium phosphate 50 mM

conditions_1: ionic strength: 0.05 M; pH: 7.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC	not available	isotropic	not available
HNCO	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HNCOCACB	not available	not available	not available
HBHACONH	not available	not available	not available
15N-edited NOESY and TOCSY-HSQC	not available	not available	not available
13C-edited NOESY-HSQC	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks