BMRB Entry 6529

Title:
Assignment of 1H, 13C and 15N resonances of the 2S albumin Ber e 1 from Brazilian nut
Deposition date:
2005-03-03
Original release date:
2006-04-06
Authors:
Tengel, Tobias; Alcocer, Marcos; Schleucher, Jurgen; Larsson, Goran
Citation:

Citation: Tengel, Tobias; Alcocer, Marcos; Schleucher, Jurgen; Larsson, Goran. "Complete Assignment and Secondary Structure of the Brazil Nut Allergen Ber e 1"  J. Biomol. NMR 32, 336-336 (2005).

Assembly members:

Assembly members:
2S sulfur-rich seed storage protein from Brazil nut, polymer, 114 residues, 136000 Da.

Natural source:

Natural source:   Common Name: Brazil nut   Taxonomy ID: 3645   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Bertholletia excelsa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts121
1H chemical shifts784

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ber e 11

Entities:

Entity 1, Ber e 1 114 residues - 136000 Da.

1   GLUALAGLUALAGLNGLUGLUCYSARGGLU
2   GLNMETGLNARGGLNGLNMETLEUSERHIS
3   CYSARGMETTYRMETARGGLNGLNMETGLU
4   GLUSERTHRTYRGLNTHRMETPROARGARG
5   GLYMETGLUPROHISMETSERGLUCYSCYS
6   GLUGLNLEUGLUGLYMETASPGLUSERCYS
7   ARGCYSGLUGLYLEUARGMETMETMETARG
8   METMETGLNGLNLYSGLUMETGLNPROARG
9   GLYGLUGLNMETARGARGMETMETARGLEU
10   ALAGLUASNILEPROSERARGCYSASNLEU
11   SERPROMETARGCYSPROMETGLYGLYSER
12   ILEALAGLYPHE

Samples:

sample_1: 2S sulfur-rich seed storage protein from Brazil nut, [U-13C; U-15N], 1 mM; Sodium phosphate buffer 20 mM; Sodium azide 1 mM; H2O 10%; D2O 90%

sample_2: 2S sulfur-rich seed storage protein from Brazil nut, [U-13C; U-15N], 1 mM; Sodium phosphate buffer 20 mM; Sodium azide 1 mM; D2O 100%

sample_3: 2S sulfur-rich seed storage protein from Brazil nut, [U-15N], 1 mM; Sodium phosphate buffer 20 mM; Sodium azide 1 mM; H2O 10%; D2O 90%

conditions_1: pH: 5.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
13C-NOESYnot availableisotropicnot available
15N-NOESYnot availableisotropicnot available
CBCACONHnot availableisotropicnot available
CBCANHnot availableisotropicnot available
HACANHnot availableisotropicnot available
HBHACONHnot availableisotropicnot available
hCCCONHnot availablenot availablenot available
HNCOnot availableisotropicnot available
HccCONHnot availablenot availablenot available
HNHAnot availableisotropicnot available
LRCHnot availableisotropicnot available

Software:

NMRDraw v2.2, . -

NMR spectrometers:

  • Bruker DRX (cryo probe) 600 MHz

Related Database Links:

PDB
DBJ BAA96554
GB ACI70207
PRF 1905414A

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks