BMRB Entry 6524

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6524

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Title:
ApoE N-terminal domain
Deposition date:
2005-02-24
Original release date:
2005-07-26
Authors:
Xu, Chao; Sivashanmugam, Arun; Hoyt, David; Wang, Jianjun
Citation:

Citation: Xu, Chao; Sivashanmugam, Arun; Hoyt, David; Wang, Jianjun. "A complete backbone assignment of the apolipoprotein E LDL receptor binding domain"  J. Biomol. NMR 32, 177-177 (2005).

Assembly members:

Assembly members:
human apolipoprotein E N-terminal domain, polymer, 183 residues, 21323 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts348
15N chemical shifts175
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoE N-terminal domain1

Entities:

Entity 1, apoE N-terminal domain 183 residues - 21323 Da.

1   LYSVALGLUGLNALAVALGLUTHRGLUPRO
2   GLUPROGLULEUARGGLNGLNTHRGLUTRP
3   GLNSERGLYGLNARGTRPGLULEUALALEU
4   GLYARGPHETRPASPTYRLEUARGTRPVAL
5   GLNTHRLEUSERGLUGLNVALGLNGLUGLU
6   LEULEUSERSERGLNVALTHRGLNGLULEU
7   ARGALALEUMETASPGLUTHRMETLYSGLU
8   LEULYSALATYRLYSSERGLULEUGLUGLU
9   GLNLEUTHRPROVALALAGLUGLUTHRARG
10   ALAARGLEUSERLYSGLULEUGLNALAALA
11   GLNALAARGLEUGLYALAASPMETGLUASP
12   VALCYSGLYARGLEUVALGLNTYRARGGLY
13   GLUVALGLNALAMETLEUGLYGLNSERTHR
14   GLUGLULEUARGVALARGLEUALASERHIS
15   LEUARGLYSLEUARGLYSARGLEULEUARG
16   ASPALAASPASPLEUGLNLYSARGLEUALA
17   VALTYRGLNALAGLYALAARGGLUGLYALA
18   GLUARGGLYLEUSERALAILEARGGLUARG
19   LEUGLYPRO

Samples:

sample_1: apoE N-terminal domain, [U-13C; U-15N; U-70% 2H], 1 mM; Sodium Phosphate 100 mM; NaN3 1 mM; DSS 0.05 mM; EDTA 10 mM; d-DTT 90 mM

sample_2: apoE N-terminal domain, [U-13C; U-15N; U-35% 2H], 1 mM; Sodium Phosphate 100 mM; NaN3 1 mM; DSS 0.06 mM; EDTA 10 mM; d-DTT 80 mM

conditions_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HN(CO)CAsample_1isotropicconditions_1
HNCAsample_1isotropicconditions_1
HN(COCA)CBsample_1isotropicconditions_1
HNCACBsample_1isotropicconditions_1
3D 15N-edited NOESYsample_2isotropicconditions_1

Software:

NMRPipe -

PIPP -

NMRView -

NMR spectrometers:

  • Varian INOVA 500 MHz MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15744
PDB
DBJ BAG37412 BAG72927
EMBL CAA25017
GB AAB59397 AAB59518 AAB59546 AAD02505 AAG27089
PRF 1506383A
REF NP_000032 NP_001289617 NP_001289618 NP_001289619 NP_001289620
SP P02649
AlphaFold P02649

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks