BMRB Entry 6520

Name: 1H, 15N Chemical shift assignments for recombinant elicitor protein PcF from the oomycete pathogen P. cactorum
Published: 2009-05-27

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PDB ID: 2bic
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6520
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N Chemical shift assignments for recombinant elicitor protein PcF from the oomycete pathogen P. cactorum

Deposition date:

2005-02-21

Original release date:

2009-05-27

Authors:

Nicastro, Giuseppe; Orsomando, Giuseppe; Desario, F.; Ferrari, Elena; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio

Citation:

Citation: Nicastro, G.; Orsomando, Giuseppe; Desario, F.; Ferrari, E.; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio. "The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum displays a helix-loop-helix fold and reveals calcium binding ability" .

Assembly members:

Assembly members:
elicitor protein Pcf from the oomycete pathogen P. cactorum, polymer, 52 residues, 6214 Da.

Natural source:

Natural source: Common Name: Pichia Pastoris Taxonomy ID: 4922 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phytophthora cactorum

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
elicitor protein Pcf from the oomycete pathogen P. cactorum: EDPLYCQAIGCPTLYSEANL AVSKECRDQGKLGDDFHRCC EEQCGSTTPASA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
15N chemical shifts	49
1H chemical shifts	321

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pcf monomer	1

Entities:

Entity 1, Pcf monomer 52 residues - 6214 Da.

1

GLU

ASP

PRO

LEU

TYR

CYS

GLN

ALA

ILE

GLY

2

CYS

PRO

THR

LEU

TYR

SER

GLU

ALA

ASN

LEU

3

ALA

VAL

SER

LYS

GLU

CYS

ARG

ASP

GLN

GLY

4

LYS

LEU

GLY

ASP

PHE

HIS

ARG

CYS

5

GLU

GLN

CYS

GLY

SER

THR

PRO

ALA

6

SER

ALA

Samples:

sample_1: Pcf monomer, [U-15N], 0.65 mM

conditions_1: pH: 5.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D-TOCSY-HSQC	not available	not available	not available
3D-NOESY-HSQC	not available	not available	not available
2D-NOESY	not available	not available	not available
2D-TOSCY	not available	not available	not available
2D-HSQC-15N	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2BIC
GB	AAK63068