BMRB Entry 6512

Name: 1H, 15N, and 13C resonance assignments of Vaccinia Virus Envelope protein, A27L
Published: 2005-07-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6512

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, and 13C resonance assignments of Vaccinia Virus Envelope protein, A27L

Deposition date:

2005-02-18

Original release date:

2005-07-26

Authors:

Chu, Feng-I

Citation:

Citation: Chu, Feng-I; Ho, Yu; Tzou, Der-Lii. "NMR assignment of the vaccinia virus envelope protein A27L" J. Biomol. NMR 32, 178-178 (2005).

Assembly members:

Assembly members:
A27L vaccinia virus envelope protein, polymer, 137 residues, 15509 Da.

Natural source:

Natural source: Common Name: Vaccinia virus Taxonomy ID: 10245 Superkingdom: Viruses Kingdom: not available Genus/species: Poxviridae Vaccinia virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A27L vaccinia virus envelope protein: MASMTGGQQMGRGSMDGTLF PGDDDLAIPATEFFSTKAAK KPEAKREAIVKADEDDNEET LKQRLTNLEKKITNVTTKFE QIEKCCKRNDEVLFRLENHA ETLRAAMISLAKKIDVQTGR RQYEKLAAALEHHHHHH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	487
15N chemical shifts	124
1H chemical shifts	564

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	A27L, chain 1	1
2	A27L, chain 2	1
3	A27L, chain 3	1

Entities:

Entity 1, A27L, chain 1 137 residues - 15509 Da.

1

MET

ALA

SER

MET

THR

GLY

GLN

MET

2

GLY

ARG

GLY

SER

MET

ASP

GLY

THR

LEU

PHE

3

PRO

GLY

ASP

LEU

ALA

ILE

PRO

ALA

4

THR

GLU

PHE

SER

THR

LYS

ALA

LYS

5

LYS

PRO

GLU

ALA

LYS

ARG

GLU

ALA

ILE

VAL

6

LYS

ALA

ASP

GLU

ASP

ASN

GLU

THR

7

LEU

LYS

GLN

ARG

LEU

THR

ASN

LEU

GLU

LYS

8

LYS

ILE

THR

ASN

VAL

THR

LYS

PHE

GLU

9

GLN

ILE

GLU

LYS

CYS

LYS

ARG

ASN

ASP

10

GLU

VAL

LEU

PHE

ARG

LEU

GLU

ASN

HIS

ALA

11

GLU

THR

LEU

ARG

ALA

MET

ILE

SER

LEU

12

ALA

LYS

ILE

ASP

VAL

GLN

THR

GLY

ARG

13

ARG

GLN

TYR

GLU

LYS

LEU

ALA

LEU

14

GLU

HIS

Samples:

sample_1: A27L vaccinia virus envelope protein, [U-99% 13C; U-99% 15N], 1.5 mM

Ex-cond_1: pH: 3.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
CBCANH	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HBHA(CBCACO)NH	not available	not available	not available
1H-15N-TOCSY-HSQC	not available	not available	not available
3D-HCCCONH	not available	not available	not available
3D-CCCONH	not available	not available	not available
2D-HSQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker AVANCE 600 MHz

EMBL	CAA46494 CAA53001 CAA53856 CAB11754 CAB11756
GB	AAA48152 AAA48248 AAA60882 AAB96472 AAF34032
REF	NP_619946 YP_233032
SP	P11258 P20535
AlphaFold	P11258 P20535