BMRB Entry 6512

Title:
1H, 15N, and 13C resonance assignments of Vaccinia Virus Envelope protein, A27L
Deposition date:
2005-02-18
Original release date:
2005-07-26
Authors:
Chu, Feng-I
Citation:

Citation: Chu, Feng-I; Ho, Yu; Tzou, Der-Lii. "NMR assignment of the vaccinia virus envelope protein A27L"  J. Biomol. NMR 32, 178-178 (2005).

Assembly members:

Assembly members:
A27L vaccinia virus envelope protein, polymer, 137 residues, 15509 Da.

Natural source:

Natural source:   Common Name: Vaccinia virus   Taxonomy ID: 10245   Superkingdom: Viruses   Kingdom: not available   Genus/species: Poxviridae Vaccinia virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21

Experimental source:

Natural source:   Common Name: Vaccinia virus   Taxonomy ID: 10245   Superkingdom: Viruses   Kingdom: not available   Genus/species: Poxviridae Vaccinia virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts124
1H chemical shifts564

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A27L, chain 11
2A27L, chain 21
3A27L, chain 31

Entities:

Entity 1, A27L, chain 1 137 residues - 15509 Da.

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGGLYSERMETASPGLYTHRLEUPHE
3   PROGLYASPASPASPLEUALAILEPROALA
4   THRGLUPHEPHESERTHRLYSALAALALYS
5   LYSPROGLUALALYSARGGLUALAILEVAL
6   LYSALAASPGLUASPASPASNGLUGLUTHR
7   LEULYSGLNARGLEUTHRASNLEUGLULYS
8   LYSILETHRASNVALTHRTHRLYSPHEGLU
9   GLNILEGLULYSCYSCYSLYSARGASNASP
10   GLUVALLEUPHEARGLEUGLUASNHISALA
11   GLUTHRLEUARGALAALAMETILESERLEU
12   ALALYSLYSILEASPVALGLNTHRGLYARG
13   ARGGLNTYRGLULYSLEUALAALAALALEU
14   GLUHISHISHISHISHISHIS

Samples:

sample_1: A27L vaccinia virus envelope protein, [U-99% 13C; U-99% 15N], 1.5 mM

Ex-cond_1: pH: 3.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
1H-15N-TOCSY-HSQCnot availablenot availablenot available
3D-HCCCONHnot availablenot availablenot available
3D-CCCONHnot availablenot availablenot available
2D-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

EMBL CAA46494 CAA53001 CAA53856 CAB11754 CAB11756
GB AAA48152 AAA48248 AAA60882 AAB96472 AAF34032
REF NP_619946 YP_233032
SP P11258 P20535

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks