BMRB Entry 6505
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6505
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Aramini, James; Rossi, Paolo; Anklin, Clemens; Xiao, Rong; Montelione, Gaetano. "Microgram-scale protein structure determination by NMR" Nature Methods 4, 491-493 (2007).
PubMed: 17496898
Assembly members:
MM1357, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: Methanosarcina mazei Taxonomy ID: 2209 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina mazei
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MM1357: MFREESRSVPVEEGEVYDVT
IQDIARQGDGIARIEGFVIF
VPGTKVGDEVRIKVERVLPK
FAFASVVE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 482 |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hypothetical protein MM1357 | 1 |
Entities:
Entity 1, hypothetical protein MM1357 68 residues - Formula weight is not available
| 1 | MET | PHE | ARG | GLU | GLU | SER | ARG | SER | VAL | PRO | ||||
| 2 | VAL | GLU | GLU | GLY | GLU | VAL | TYR | ASP | VAL | THR | ||||
| 3 | ILE | GLN | ASP | ILE | ALA | ARG | GLN | GLY | ASP | GLY | ||||
| 4 | ILE | ALA | ARG | ILE | GLU | GLY | PHE | VAL | ILE | PHE | ||||
| 5 | VAL | PRO | GLY | THR | LYS | VAL | GLY | ASP | GLU | VAL | ||||
| 6 | ARG | ILE | LYS | VAL | GLU | ARG | VAL | LEU | PRO | LYS | ||||
| 7 | PHE | ALA | PHE | ALA | SER | VAL | VAL | GLU |
Samples:
sample_1: MM1357, [U-13C; U-15N], 2 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H2O 95%
sample_2: MM1357, [U-13C; U-15N], 0.56 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 100%
sample_3: MM1357, [U-5% 13C; U-15N], 0.78 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H2O 95%
sample_cond_1: pH: 6.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY (aliph) | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| Hi-res CH-HSQC | not available | not available | sample_cond_1 |
| HcCH-COSY | not available | not available | sample_cond_1 |
| Hi-res CH-HSQC (stereo V and L Meth.) | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| HCCH-TOCSY (aliph) | not available | not available | sample_cond_1 |
| HCCH-TOCSY (arom) | not available | not available | sample_cond_1 |
| HNCOCACB | not available | not available | sample_cond_1 |
| HNCO | not available | not available | sample_cond_1 |
| HNCA | not available | not available | sample_cond_1 |
| HBHANH | not available | not available | sample_cond_1 |
| HNCOCA | not available | not available | sample_cond_1 |
| HBHACONH | not available | not available | sample_cond_1 |
| HNCACO | not available | not available | sample_cond_1 |
| HNCACB | not available | not available | sample_cond_1 |
| 3D 13C-separated NOESY (arom) | not available | not available | sample_cond_1 |
Software:
VNMR v6.1C - collection
XWINNMR v3.5 - collection
NMRPipe v2.1 - processing
Sparky v3.91 - data analysis
AutoAssign v1.14 - data analysis
AutoStructure v2.1.0 - refinement
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| GB | AAM03512 AAM31053 AAZ69994 AAZ69995 |
| REF | NP_615032 NP_633381 WP_011020117 WP_011033303 WP_011306043 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks