BMRB Entry 6502

Name: DNA binding domain in XPF/ERCC-1 complex
Published: 2006-02-17

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6502

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

DNA binding domain in XPF/ERCC-1 complex

Deposition date:

2005-02-16

Original release date:

2006-02-17

Authors:

Masuyama, Mika; Ishino, Sonoko; Nishino, Tatsuya; Moriuchi, Hiroshi; Ueno, Hiroshi; Morikawa, Kousuke; Tate, Shin-ichi

Citation:

Citation: Masuyama, Mika; Ishino, Sonoko; Nishino, Tatsuya; Moriuchi, Hiroshi; Ueno, Hiroshi; Morikawa, Kousuke; Tate, Shin-ichi. "Resonance assignments for the DNA binding domain of ERCC-1/XPF heterodimer" J. Biomol. NMR 32, 175-175 (2005).

Assembly members:

Assembly members:
XPF subunit, polymer, 100 residues, Formula weight is not available
ERCC-1 subunit, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
XPF subunit: GSHMPQPDAATALAITADSE TLPESEKYNPGPQDFLLKMP GVNAKNCRSLMHHVKNIAEL AALSQDELTSILGNAANAKQ LYDFIHTSFAEVVSKGKGKK
ERCC-1 subunit: GSHMPADLLMEKLEQDFVSR VTECLTTVKSVNKTDSQTLL TTFGSLEQLIAASREDLALC PGLGPQKARRLFDVLHEPFL KVP

Data sets:

assigned_chemical_shifts
- assign_1

Data type	Count
13C chemical shifts	622
15N chemical shifts	172
1H chemical shifts	1085

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	XPF subunit	1
2	ERCC-1 subunit	2

Entities:

Entity 1, XPF subunit 100 residues - Formula weight is not available

number 1-100 corresponds to XPF subunit. In each fragment, the first four residues (GSHM) are artificially added from expression vector.

1	GLY	SER	HIS	MET	PRO	GLN	PRO	ASP	ALA	ALA
2	THR	ALA	LEU	ALA	ILE	THR	ALA	ASP	SER	GLU
3	THR	LEU	PRO	GLU	SER	GLU	LYS	TYR	ASN	PRO
4	GLY	PRO	GLN	ASP	PHE	LEU	LEU	LYS	MET	PRO
5	GLY	VAL	ASN	ALA	LYS	ASN	CYS	ARG	SER	LEU
6	MET	HIS	HIS	VAL	LYS	ASN	ILE	ALA	GLU	LEU
7	ALA	ALA	LEU	SER	GLN	ASP	GLU	LEU	THR	SER
8	ILE	LEU	GLY	ASN	ALA	ALA	ASN	ALA	LYS	GLN
9	LEU	TYR	ASP	PHE	ILE	HIS	THR	SER	PHE	ALA
10	GLU	VAL	VAL	SER	LYS	GLY	LYS	GLY	LYS	LYS

Entity 2, ERCC-1 subunit 83 residues - Formula weight is not available

In each fragment, the first four residues (GSHM) are artificially added from expression vector.

1

GLY

SER

HIS

MET

PRO

ALA

ASP

LEU

MET

2

GLU

LYS

LEU

GLU

GLN

ASP

PHE

VAL

SER

ARG

3

VAL

THR

GLU

CYS

LEU

THR

VAL

LYS

SER

4

VAL

ASN

LYS

THR

ASP

SER

GLN

THR

LEU

5

THR

PHE

GLY

SER

LEU

GLU

GLN

LEU

ILE

6

ALA

SER

ARG

GLU

ASP

LEU

ALA

LEU

CYS

7

PRO

GLY

LEU

GLY

PRO

GLN

LYS

ALA

ARG

8

LEU

PHE

ASP

VAL

LEU

HIS

GLU

PRO

PHE

LEU

9

LYS

VAL

PRO

Samples:

sample_1: XPF subunit, [U-95% 13C; U-90% 15N], 1.4 ± 0.1 mM; ERCC-1 subunit, [U-95% 13C; U-90% 15N], 1.4 ± 0.1 mM; sodium phosphate buffer 50 mM

condition_1: ionic strength: 50 mM; pH*: 6.4; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

PDB	1Z00 2A1J
DBJ	BAG37398 BAG52472
GB	AAA35810 AAA52394 AAC16253 AAH08930 AAH52813
PRF	1403276A
REF	NP_001069853 NP_001152781 NP_001181860 NP_001974 NP_973730
SP	P07992 Q1LZ75
TPG	DAA19632
AlphaFold	P07992 Q1LZ75