BMRB Entry 6500

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SIP (74-178)
Published: 2005-08-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6500

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for SIP (74-178)

Deposition date:

2005-02-14

Original release date:

2005-08-16

Authors:

Bhattacharya, Shibani; Lee, Young-Tae; Michowski, Wojciech; Filipek, Anna; Kuznicki, Jacek; Chazin, Walter

Citation:

Citation: Bhattacharya, Shibani; Lee, Young-Tae; Michowski, Wojciech; Filipek, Anna; Kuznicki, Jacek; Chazin, Walter. "The Modular Structure of SIP Facilitates Its Role in Stabilizing Multiprotein Assemblies" Biochemistry 44, 9462-9471 (2005).
PubMed: 15996101

Assembly members:

Assembly members:
SIP (74-178), polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SIP (74-178): VKISNYGWDQSDKFVKIYIT LTGVHQVPTENVQVHFTERS FDLLVKNLNGKNYSMIVNNL LKPISVESSSKKVKTDTVII LCRKKAENTRWDYLTQVEKE CKEKE

Data sets:

assigned_chemical_shifts
- CSR_1

Data type	Count
13C chemical shifts	279
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SIP CS-Domain	1

Entities:

Entity 1, SIP CS-Domain 105 residues - Formula weight is not available

1

VAL

LYS

ILE

SER

ASN

TYR

GLY

TRP

ASP

GLN

2

SER

ASP

LYS

PHE

VAL

LYS

ILE

TYR

ILE

THR

3

LEU

THR

GLY

VAL

HIS

GLN

VAL

PRO

THR

GLU

4

ASN

VAL

GLN

VAL

HIS

PHE

THR

GLU

ARG

SER

5

PHE

ASP

LEU

VAL

LYS

ASN

LEU

ASN

GLY

6

LYS

ASN

TYR

SER

MET

ILE

VAL

ASN

LEU

7

LEU

LYS

PRO

ILE

SER

VAL

GLU

SER

8

LYS

VAL

LYS

THR

ASP

THR

VAL

ILE

9

LEU

CYS

ARG

LYS

ALA

GLU

ASN

THR

ARG

10

TRP

ASP

TYR

LEU

THR

GLN

VAL

GLU

LYS

GLU

11

CYS

LYS

GLU

LYS

GLU

Samples:

Sample_1: SIP (74-178), [U-100% 13C; U-100% 15N], 1.0 mM; NaPi 20 mM; NaCl 50 mM; DTT 5 mM; H2O 90%; D2O 10%

Exp_cond_1: pH: 7.0; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C CT-HSQC	not available	not available	not available
3D-HNCO	not available	not available	not available
3D-HNCACB	not available	not available	not available
3D-CBCA(CO)NH	not available	not available	not available

Software:

FELIX v2000 - analysis, processing

NMR spectrometers:

Bruker AVANCE 600 MHz

GB	AAC16757 AAH25948 AAH79007 EDL39341 EDM09438
REF	NP_001004208 NP_033916 XP_002928087 XP_003130369 XP_003497720
SP	Q6AYK6 Q9CXW3
AlphaFold	Q6AYK6 Q9CXW3