BMRB Entry 6499

Name: NMR structure of antimicrobial peptide distinctin in water
Published: 2005-05-26

Click here to enlarge.

PDB ID: 1xkm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6499
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR structure of antimicrobial peptide distinctin in water

Deposition date:

2005-02-09

Original release date:

2005-05-26

Authors:

Amodeo, P.; Raimondo, D.; Andreotti, G.; Motta, A.; Scaloni, A.

Citation:

Citation: Raimondo, D.; Andreotti, G.; Saint, N.; Amodeo, P.; Renzone, G.; Sanseverino, M.; Zocchi, I.; Molle, G.; Motta, A.; Scaloni, A.. "A folding-dependent mechanism of antimicrobial peptide resistance to degradation unveiled by solution structure of distinctin." Proc. Natl. Acad. Sci. U. S. A. ., .-..
PubMed: 15840728

Assembly members:

Assembly members:
Distinctin chain A, polymer, 22 residues, Formula weight is not available
Distinctin chain B, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source: Common Name: tree frog Taxonomy ID: 164618 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Phyllomedusa distincta

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Distinctin chain A: ENREVPPGFTALIKTLRKCK II
Distinctin chain B: NLVSGLIEARKYLEQLHRKL KNCKV

Data sets:

assigned_chemical_shifts
- chemical_shift_ch_A
- chemical_shift_ch_B

Data type	Count
1H chemical shifts	413

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Distinctin chain A monomer 1	1
2	Distinctin chain B monomer 1	2
3	Distinctin chain A monomer 2	1
4	Distinctin chain B monomer 2	2

Entities:

Entity 1, Distinctin chain A monomer 1 22 residues - Formula weight is not available

1

GLU

ASN

ARG

GLU

VAL

PRO

GLY

PHE

THR

2

ALA

LEU

ILE

LYS

THR

LEU

ARG

LYS

CYS

LYS

3

ILE

Entity 2, Distinctin chain B monomer 1 25 residues - Formula weight is not available

1

ASN

LEU

VAL

SER

GLY

LEU

ILE

GLU

ALA

ARG

2

LYS

TYR

LEU

GLU

GLN

LEU

HIS

ARG

LYS

LEU

3

LYS

ASN

CYS

LYS

VAL

Samples:

sample_1: Distinctin chain A0.05 – 3.8 mM; Distinctin chain B0.05 – 3.8 mM; Phosphate 20 mM; NaCl 100 mM

sample_cond_1: ionic strength: 0.15 M; pH: 5.8; pressure: 1 atm; temperature: 300 K

sample_cond_2: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available

Software:

AMBER v5 - refinement, structure solution

Molmol v2K.2 - data analysis

xwinnmr v3.5 - processing

NMR spectrometers:

Bruker DRX 500 MHz