BMRB Entry 6494

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6494

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chorismate Mutase
Deposition date:
2005-02-08
Original release date:
2011-08-11
Authors:
Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin
Citation:

Citation: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin. "Investigation of Ligand Binding and Protein Dynamics in Bacillus subtilis Chorismate Mutase by Transverse Relaxation Optimized Spectroscopy-Nuclear Magnetic Resonance"  Biochemistry 44, 6788-6799 (2005).
PubMed: 15865424

Assembly members:

Assembly members:
BsCM, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BsCM: MMIRGIRGATTVERDTEEEI LQKTKQLLEKIIEENHTKPE DVVQMLLSATPDLHAVFPAK AVRELSGWQYVPVTCMQEMD VTGGLKKCIRVMMTVQTDVP QDQIRHVYLEKAVVLRPDLS LTKNTEL

Data sets:
Data typeCount
1H chemical shifts245
13C chemical shifts354
15N chemical shifts118
T1 relaxation values234
T2 relaxation values231

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BsCM polypeptide_A1
2BsCM polypeptide_B1
3BsCM polypeptide_C1

Entities:

Entity 1, BsCM polypeptide_A 127 residues - Formula weight is not available

1   METMETILEARGGLYILEARGGLYALATHR
2   THRVALGLUARGASPTHRGLUGLUGLUILE
3   LEUGLNLYSTHRLYSGLNLEULEUGLULYS
4   ILEILEGLUGLUASNHISTHRLYSPROGLU
5   ASPVALVALGLNMETLEULEUSERALATHR
6   PROASPLEUHISALAVALPHEPROALALYS
7   ALAVALARGGLULEUSERGLYTRPGLNTYR
8   VALPROVALTHRCYSMETGLNGLUMETASP
9   VALTHRGLYGLYLEULYSLYSCYSILEARG
10   VALMETMETTHRVALGLNTHRASPVALPRO
11   GLNASPGLNILEARGHISVALTYRLEUGLU
12   LYSALAVALVALLEUARGPROASPLEUSER
13   LEUTHRLYSASNTHRGLULEU

Samples:

sample_1: BsCM, [U-13C; U-15N; U-2H], mM

sample_2: BsCM, [U-13C; U-15N; U-35% 2H], mM

sample_3: BsCM, [U-15N], mM

conditions_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
TROSY-CT-HNCAnot availablenot availablenot available
TROSY-HNCACBnot availablenot availablenot available
TROSY-HNCAnot availablenot availablenot available
TROSY-CBCACONHnot availablenot availablenot available
NOESY-TROSYnot availablenot availablenot available
TOCSY-TROSYnot availablenot availablenot available
MQ-HACACOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • . . . MHz

Related Database Links:

REF ZP_03600723 ZP_03592031 ZP_03596312 NP_390150
SP P19080
GB ABN13175 ABN05305 ABN05308 AAA20861 AAA22249
DBJ BAI85767
EMBL CAB14185
PDB
BMRB 6495 6496

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks