BMRB Entry 6487
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6487
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ottiger, Marcel; Bax, Ad. "Determination of Relative N-HN, N-C', CA-C', and CA-HA Effective Bond Length in a Protein by NMR in a Dilute Liquid Crystalline Phase" J. Am. Chem. Soc. 120, 12334-12341 (1998).
Assembly members:
Ubiquitin, polymer, 76 residues, 8579.94 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
Ubiquitin: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG
| Data type | Count |
| residual dipolar couplings | 632 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin | 1 |
Entities:
Entity 1, Ubiquitin 76 residues - 8579.94 Da.
| 1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin, [U-13C; U-15N], 0.7 mM; DMPC/DHCP (3.0:1 molar ration) 50 mg/ml; phosphate 10 mM; H20 93%; D2O 7%
sample_2: Ubiquitin, [U-13C; U-15N], 0.7 mM; DMPC:DHCP:CTAB (30:10:1 molar ratio) 50 mg/ml; phosphate 10 mM; H20 93%; D2O 7%
conditions_1: pH: 6.6
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz