BMRB Entry 6476

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PDB ID: 1yqa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6476
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Full 1H, 13C and 15N Chemical Shift Assignments for the GII loop mutant domain (GII-L) of the yeast linker histone, Hho1p

Deposition date:

2005-02-01

Original release date:

2005-09-22

Authors:

Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean

Citation:

Citation: Sanderson, Andrew; Stott, Katherine; Stevens, Timothy; Thomas, Jean. "Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p." J. Mol. Biol. 349, 608-620 (2005).
PubMed: 15878177

Assembly members:

Assembly members:
GII-L, polymer, 87 residues, 9537 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GII-L: KASSPSSLTYKEMILKSMPQ LNDGKGSSRIVLKKYVKDTY PIVGSASNFDYLFNSAIKKC VENGELVQPKGPSGIIKLNK KKVKLST

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	403
15N chemical shifts	88
1H chemical shifts	636

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GII-L	1

Entities:

Entity 1, GII-L 87 residues - 9537 Da.

1

LYS

ALA

SER

PRO

SER

LEU

THR

TYR

2

LYS

GLU

MET

ILE

LEU

LYS

SER

MET

PRO

GLN

3

LEU

ASN

ASP

GLY

LYS

GLY

SER

ARG

ILE

4

VAL

LEU

LYS

TYR

VAL

LYS

ASP

THR

TYR

5

PRO

ILE

VAL

GLY

SER

ALA

SER

ASN

PHE

ASP

6

TYR

LEU

PHE

ASN

SER

ALA

ILE

LYS

CYS

7

VAL

GLU

ASN

GLY

GLU

LEU

VAL

GLN

PRO

LYS

8

GLY

PRO

SER

GLY

ILE

LYS

LEU

ASN

LYS

9

LYS

VAL

LYS

LEU

SER

THR

Samples:

sample_1: GII-L, [U-13C; U-15N], 1 mM; sodium phosphate buffer 100 mM; EDTA 1 mM; DTT, [U-2H], 1 – 2 mM

conditions_1: pH: 7.0; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
CBCACONH	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
(H)CC(CO)NH-TOCSY	sample_1	not available	conditions_1
TOCSY-15N-HSQC	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
NOESY-15N-HSQC	sample_1	not available	conditions_1
NOESY-13C-HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Burker DRX 500 MHz