BMRB Entry 6475

Name: 1H, 13C and 15N Chemical Shift assignments for Man5C-CBM35 from C. japonicus
Published: 2006-11-13

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PDB ID: 2bgo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6475
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift assignments for Man5C-CBM35 from C. japonicus

Deposition date:

2005-01-31

Original release date:

2006-11-13

Authors:

Tunnicliffe, Richard; Williamson, Mike

Citation:

Citation: Tunnicliffe, Richard; Bolam, David; Pell, Gavin; Gilbert, Harry; Williamson, Mike. "Structure of a Mannan-specific Family 35 Carbohydrate-Binding Module: Evidence for Significant Conformational Changes upon Ligand Binding" J. Mol. Biol. 347, 287-296 (2005).
PubMed: 15740741

Assembly members:

Assembly members:
Man5C-CBM35, polymer, 132 residues, 14933.7 Da.

Natural source:

Natural source: Common Name: Cellvibrio japonicus Taxonomy ID: 155077 Superkingdom: Bacteria Kingdom: not available Genus/species: Cellvibrio Cellvibrio japonicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia Coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Man5C-CBM35: MAVPEGNSWTYTAASASITA PAQLVGNVGELQGAGSAVIW NVDVPVTGEYRINLTWSSPY SSKVNTLVMDGTALSYAFAE ATVPVTYVQTKTLSAGNHSF GVRVGSSDWGYMNVHSLKLE LLGGLTIRSPAN

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
1H chemical shifts	835
13C chemical shifts	524
15N chemical shifts	133

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Man5C-CBM35	1

Entities:

Entity 1, Man5C-CBM35 132 residues - 14933.7 Da.

1

MET

ALA

VAL

PRO

GLU

GLY

ASN

SER

TRP

THR

2

TYR

THR

ALA

SER

ALA

SER

ILE

THR

ALA

3

PRO

ALA

GLN

LEU

VAL

GLY

ASN

VAL

GLY

GLU

4

LEU

GLN

GLY

ALA

GLY

SER

ALA

VAL

ILE

TRP

5

ASN

VAL

ASP

VAL

PRO

VAL

THR

GLY

GLU

TYR

6

ARG

ILE

ASN

LEU

THR

TRP

SER

PRO

TYR

7

SER

LYS

VAL

ASN

THR

LEU

VAL

MET

ASP

8

GLY

THR

ALA

LEU

SER

TYR

ALA

PHE

ALA

GLU

9

ALA

THR

VAL

PRO

VAL

THR

TYR

VAL

GLN

THR

10

LYS

THR

LEU

SER

ALA

GLY

ASN

HIS

SER

PHE

11

GLY

VAL

ARG

VAL

GLY

SER

ASP

TRP

GLY

12

TYR

MET

ASN

VAL

HIS

SER

LEU

LYS

LEU

GLU

13

LEU

GLY

LEU

THR

ILE

ARG

SER

PRO

14

ALA

ASN

Samples:

sample_1: Man5C-CBM35, [U-95% 13C; U-95% 15N], 1.0 mM; sodium phosphate 50 mM

conditions_1: pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC (15N and 13C)	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HN(CA)CO	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HBHA(CBCACO)NH	sample_1	not available	conditions_1
CCH-TOCSY	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
15N,13C-edited NOESY	sample_1	not available	conditions_1

Software:

Felix v2000, Accelrys - Processing, analysis

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 500 MHz
Bruker DRX 600 MHz

PDB	2BGO 2BGP
GB	AAO31761 ACE82655
REF	WP_012489045 YP_001983923