BMRB Entry 6465
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6465
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Parsons, Lisa; Orban, John. "NMR assignment of the periplasmic domain of peptidoglycan-associated
lipoprotein (Pal) from Haemophilus influenzae" J. Biomol. NMR 32, 93-93 (2005).
Assembly members:
PAL - Peptidoglycan associated lipoprotein, polymer, 134 residues, Formula weight is not available
UDP-MurNAc-pentapeptide(UYP), polymer, 7 residues, Formula weight is not available
Natural source: Common Name: H. influenzae Taxonomy ID: 727 Superkingdom: Bacteria Kingdom: not available Genus/species: Haemophilus influenzae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PAL - Peptidoglycan associated lipoprotein: CSSSNNDAAGNGAAQTFGGY
SVADLQQRYNTVYFGFDKYD
ITGEYVQILDAHAAYLNATP
AAKVLVEGNTDERGTPEYNI
ALGQRRADAVKGYLAGKGVD
AGKLGTVSYGEEKPAVLGHD
EAAYSKNRRAVLAY
UDP-MurNAc-pentapeptide(UYP): XXAXXXX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 503 |
| 1H chemical shifts | 831 |
| 15N chemical shifts | 137 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAL - Peptidoglycan associated lipoprotein | 1 |
| 2 | UDP-MurNAc-pentapeptide(UYP) | 2 |
Entities:
Entity 1, PAL - Peptidoglycan associated lipoprotein 134 residues - Formula weight is not available
| 1 | CYS | SER | SER | SER | ASN | ASN | ASP | ALA | ALA | GLY | ||||
| 2 | ASN | GLY | ALA | ALA | GLN | THR | PHE | GLY | GLY | TYR | ||||
| 3 | SER | VAL | ALA | ASP | LEU | GLN | GLN | ARG | TYR | ASN | ||||
| 4 | THR | VAL | TYR | PHE | GLY | PHE | ASP | LYS | TYR | ASP | ||||
| 5 | ILE | THR | GLY | GLU | TYR | VAL | GLN | ILE | LEU | ASP | ||||
| 6 | ALA | HIS | ALA | ALA | TYR | LEU | ASN | ALA | THR | PRO | ||||
| 7 | ALA | ALA | LYS | VAL | LEU | VAL | GLU | GLY | ASN | THR | ||||
| 8 | ASP | GLU | ARG | GLY | THR | PRO | GLU | TYR | ASN | ILE | ||||
| 9 | ALA | LEU | GLY | GLN | ARG | ARG | ALA | ASP | ALA | VAL | ||||
| 10 | LYS | GLY | TYR | LEU | ALA | GLY | LYS | GLY | VAL | ASP | ||||
| 11 | ALA | GLY | LYS | LEU | GLY | THR | VAL | SER | TYR | GLY | ||||
| 12 | GLU | GLU | LYS | PRO | ALA | VAL | LEU | GLY | HIS | ASP | ||||
| 13 | GLU | ALA | ALA | TYR | SER | LYS | ASN | ARG | ARG | ALA | ||||
| 14 | VAL | LEU | ALA | TYR |
Entity 2, UDP-MurNAc-pentapeptide(UYP) 7 residues - Formula weight is not available
| 1 | UDP | AMU | ALA | DGN | KCX | DAL | DAL |
Samples:
sample_1: PAL - Peptidoglycan associated lipoprotein, [U-13C; U-15N], 0.8 mM; UDP-MurNAc-pentapeptide(UYP), [U-13C; U-15N], 0.8 mM; sodium phospate 50 mM; sodium chloride 50 mM; EDTA 0.05 mM
conditions_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HCCONH | sample_1 | not available | conditions_1 |
| CCONH | sample_1 | not available | conditions_1 |
| aromatic constant-time 13C-HSQC | sample_1 | not available | conditions_1 |
| 2DTOCSY | sample_1 | not available | conditions_1 |
| 2DNOESY | sample_1 | not available | conditions_1 |
| 13C-3D NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| REF | ZP_00155384 YP_001292339 YP_248096 NP_438542 YP_001290090 |
| SP | P10324 |
| GB | AAX87436 ABQ97707 AAA24994 AAC22039 AAA24940 |
| EMBL | CBW28680 |
| BMRB | 6858 |
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks