BMRB Entry 6451

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6451

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Title:
1H, 15N and 13C resonance assigment of the chaperone CesT from enteropathogenic Escherichia coli
Deposition date:
2004-12-31
Original release date:
2005-07-26
Authors:
Rumpel, Sigrun; Kim, Hai-Young; Vijayan, Vinesh; Becker, Stefan; Zweckstetter, Markus
Citation:

Citation: Rumpel, Sigrun; Kim, Hai-Young; Vijayan, Vinesh; Becker, Stefan; Zweckstetter, Markus. "Letter to the Editor: Backbone resonance assigment of the homodimeric, 35 kDa chaperone CesT from enteropathogenic Escherichia coli"  J. Biomol. NMR 31, 377-378 (2005).
PubMed: 15929012

Assembly members:

Assembly members:
chaperone for the enteropathogenic E. coli secreted protein Tir, polymer, 158 residues, 15431 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chaperone for the enteropathogenic E. coli secreted protein Tir: GHMSSRSELLLDRFAEKIGV GSISFNENRLCSFAIDEIYY ISLSDANDEYMMIYGVCGKF PTDNPNFALEILNANLWFAE NGGPYLCYESGAQSLLLALR FPLDDATPEKLENEIEVVVK SMENLYLVLHNQGITLENEH MKIEEISSSDNKHYYAGR

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts148
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CesT subunit 11
2CesT subunit 21

Entities:

Entity 1, CesT subunit 1 158 residues - 15431 Da.

1   GLYHISMETSERSERARGSERGLULEULEU
2   LEUASPARGPHEALAGLULYSILEGLYVAL
3   GLYSERILESERPHEASNGLUASNARGLEU
4   CYSSERPHEALAILEASPGLUILETYRTYR
5   ILESERLEUSERASPALAASNASPGLUTYR
6   METMETILETYRGLYVALCYSGLYLYSPHE
7   PROTHRASPASNPROASNPHEALALEUGLU
8   ILELEUASNALAASNLEUTRPPHEALAGLU
9   ASNGLYGLYPROTYRLEUCYSTYRGLUSER
10   GLYALAGLNSERLEULEULEUALALEUARG
11   PHEPROLEUASPASPALATHRPROGLULYS
12   LEUGLUASNGLUILEGLUVALVALVALLYS
13   SERMETGLUASNLEUTYRLEUVALLEUHIS
14   ASNGLNGLYILETHRLEUGLUASNGLUHIS
15   METLYSILEGLUGLUILESERSERSERASP
16   ASNLYSHISTYRTYRALAGLYARG

Samples:

sample_1: chaperone for the enteropathogenic E. coli secreted protein Tir, [U-95% 13C; U-90% 15N; 75% 2H], 0.5 – 1.5 mM; K3PO4 50 mM; NaCl 100 mM; d-DTT 1 mM; EDTA 0.5 mM; D20 10%; H20 90%

sample_2: chaperone for the enteropathogenic E. coli secreted protein Tir, [U-95% 13C; U-90% 15N], 0.5 – 1.5 mM; K3PO4 50 mM; NaCl 100 mM; d-DTT 1 mM; EDTA 0.5 mM; D20 10%; H20 90%

cond_set_1: ionic strength: 0.15 M; pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 700 MHz
  • Bruker AVANCE 900 MHz

Related Database Links:

DBJ BAE80445 BAE80446 BAE80447 BAE80448 BAE80449
EMBL CAA11066 CAC81870 CAG17537 CAI43866 CAR47989
GB AAA62774 AAB02942 AAB36673 AAB39867 AAC32029
REF WP_000098785 WP_000098786 WP_000098787 WP_000098788 WP_000098789
SP O30999 P21244 Q47015
AlphaFold O30999 P21244 Q47015

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks