BMRB Entry 6446

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PDB ID: 1y4e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6446
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural and Functional Characterization of Transmembrane Segment IV of the NHE1 Isoform of the Na+/H+ Exchanger

Deposition date:

2004-12-28

Original release date:

2005-06-02

Authors:

Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.

Citation:

Citation: Slepkov, E.; Rainey, J.; Li, X.; Liu, Y.; Cheng, F.; Lindhout, D.; Sykes, B.; Fliegel, L.. "The Na+/H+ Exchanger isoform 1" J. Biol. Chem. 280, 17863-17872 (2005).
PubMed: 15677483

Assembly members:

Assembly members:
Sodium/hydrogen exchanger 1, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sodium/hydrogen exchanger 1: FLQSDVFFLFLLPPIILDAG YFLPLRX

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	135
13C chemical shifts	52
15N chemical shifts	25

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sodium/hydrogen exchanger 1 TM segment IV	1

Entities:

Entity 1, Sodium/hydrogen exchanger 1 TM segment IV 27 residues - Formula weight is not available

1

PHE

LEU

GLN

SER

ASP

VAL

PHE

LEU

PHE

2

LEU

PRO

ILE

LEU

ASP

ALA

GLY

3

TYR

PHE

LEU

PRO

LEU

ARG

HSL

Samples:

sample_1: Sodium/hydrogen exchanger 1 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%

sample_2: Sodium/hydrogen exchanger 1, [U-15N], 2 mM; DSS 1 mM; CDCl3 44%; CD3OH 44%; H2O 11%

sample_cond_1: pH: .; temperature: 303 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1
2D NOESY	not available	not available	sample_cond_1
2D TOCSY	not available	not available	sample_cond_1

Software:

VNMR vvarious - collection

NMRPipe v2.3 - processing

SPARKY v3.109 and 3.110 - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

Varian INOVA 500 MHz

PDB	1Y4E
DBJ	BAE02258 BAE29332 BAE32882 BAE38221 BAG36869
EMBL	CAA48771
GB	AAA92976 AAA98479 AAB59460 AAC60606 AAF21350
REF	NP_001233682 NP_001273957 NP_003038 NP_036784 NP_058677
SP	P19634 P26431 P48761 Q61165
AlphaFold	P19634 P26431 P48761 Q61165