BMRB Entry 6440

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6440

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Title:
1H, 15N and 13C backbone assignments of 28kD phytochrome B PAS1-PAS2 domain
Deposition date:
2004-12-21
Original release date:
2005-04-08
Authors:
Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro
Citation:

Citation: Kobayashi, Toshitatsu; Mishima, Masaki; Akagi, Kayo; Sakai, Nobuya; Katoh, Etsuko; Takano, Makoto; Yamazaki, Toshimasa; Kojima, Chojiro. "Letter to the Editor: 1H, 15N and 13C backbone and side-chain assignments of the rice phytochrome B PAS1 domain and backbone assignments of the PAS1-PAS2 domain"  J. Biomol. NMR 31, 269-270 (2005).

Assembly members:

Assembly members:
Rice phytochrome B PAS1-PAS2 domain, polymer, 258 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rice   Taxonomy ID: 4530   Superkingdom: Eukaryota   Kingdom: Plantae   Genus/species: Oryza sativa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rice phytochrome B PAS1-PAS2 domain: VRLIETATVPIFAVDTDGCI NGWNAKVAELTGLSVEEAMG KSLVNDLIFKESEETVNKLL SRALRGDEDKNVEIKLKTFG PEQSKGPIFVIVNACSSRDY TKNIVGVCFVGQDVTGQKVV MDKFINIQGDYKAIVHNPNP LIPPIFASDENTCCSEWNTA MEKLTGWSRGEVVGKLLVGE VFGNCCRLKGPDALTKFMIV LHNAIGGQDCEKFPFSFFDK NGKYVQALLTANTRSRMDGE AIGAFCFLQIASPELQQA

Data sets:
Data typeCount
13C chemical shifts742
15N chemical shifts246
1H chemical shifts246

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rice phytochrome B PAS1-PAS2 domain1

Entities:

Entity 1, Rice phytochrome B PAS1-PAS2 domain 258 residues - Formula weight is not available

1   VALARGLEUILEGLUTHRALATHRVALPRO
2   ILEPHEALAVALASPTHRASPGLYCYSILE
3   ASNGLYTRPASNALALYSVALALAGLULEU
4   THRGLYLEUSERVALGLUGLUALAMETGLY
5   LYSSERLEUVALASNASPLEUILEPHELYS
6   GLUSERGLUGLUTHRVALASNLYSLEULEU
7   SERARGALALEUARGGLYASPGLUASPLYS
8   ASNVALGLUILELYSLEULYSTHRPHEGLY
9   PROGLUGLNSERLYSGLYPROILEPHEVAL
10   ILEVALASNALACYSSERSERARGASPTYR
11   THRLYSASNILEVALGLYVALCYSPHEVAL
12   GLYGLNASPVALTHRGLYGLNLYSVALVAL
13   METASPLYSPHEILEASNILEGLNGLYASP
14   TYRLYSALAILEVALHISASNPROASNPRO
15   LEUILEPROPROILEPHEALASERASPGLU
16   ASNTHRCYSCYSSERGLUTRPASNTHRALA
17   METGLULYSLEUTHRGLYTRPSERARGGLY
18   GLUVALVALGLYLYSLEULEUVALGLYGLU
19   VALPHEGLYASNCYSCYSARGLEULYSGLY
20   PROASPALALEUTHRLYSPHEMETILEVAL
21   LEUHISASNALAILEGLYGLYGLNASPCYS
22   GLULYSPHEPROPHESERPHEPHEASPLYS
23   ASNGLYLYSTYRVALGLNALALEULEUTHR
24   ALAASNTHRARGSERARGMETASPGLYGLU
25   ALAILEGLYALAPHECYSPHELEUGLNILE
26   ALASERPROGLULEUGLNGLNALA

Samples:

sample_1: Rice phytochrome B PAS1-PAS2 domain, [U-13C; U-15N], 0.2 mM; potassium phosphate 50 mM; potassium chloride 20 mM; dithiothreitol 5 mM; deuterium oxide, [U-2H], 10%

conditions_1: ionic strength: 0.05 M; pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N_HSQCnot availablenot availablenot available
3D-HNCACBnot availablenot availablenot available
3D-HN(CO)CACBnot availablenot availablenot available
3D-HNCOnot availablenot availablenot available
3D-HN(CA)COnot availablenot availablenot available

Software:

xwinnmr v3.5 - data aquisition

NMRPipe - data processing

SPARKY v3.106 - assignments, peak picking

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

DBJ BAC76432 BAD86669 BAF11824
EMBL CAA40795
GB ABF95558 AFK30970 AFK30971 AFK30972 AFK30973
REF NP_001049910
SP A2XFW2 Q10MG9
AlphaFold A2XFW2 Q10MG9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks