BMRB Entry 6434

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6434
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone and Sidechain Assignments of the Lambda Bacteriophage Tail Assembly Protein gpU
Deposition date:
2004-12-16
Original release date:
2005-07-26
Authors:
Edmonds, Lizbeth; Thirumoorthy, Ramanan; Liu, Amanda; Davidson, Alan; Donaldson, Logan
Citation:

Citation: Edmonds, Lizbeth; Thirumoorthy, Ramanan; Liu, Amanda; Davidson, Alan; Donaldson, Logan. "Letter to the Editor: NMR assignment of the gpU tail protein from lambda bacteriophage"  J. Biomol. NMR 32, 91-92 (2005).

Assembly members:

Assembly members:
gpU, polymer, 150 residues, 16681 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: bacteriophage lambda   Taxonomy ID: 10710   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambda-like viruses lambda

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gpU: GSSHHHHHHSSGLVPRGSHM KHTELRAAVLDALEKHDTGA TFFDGRPAVFDEADFPAVAV YLTGAEYTGEELDSDTWQAE LHIEVFLPAQVPDSELDAWM ESRIYPVMSDIPALSDLITS MVASGYDYRRDDDAGLWSSA DLTYVITYEM

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts546
1H chemical shifts854
15N chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GPU monomer1
2MG_2+2

Entities:

Entity 1, GPU monomer 150 residues - 16681 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   LYSHISTHRGLULEUARGALAALAVALLEU
4   ASPALALEUGLULYSHISASPTHRGLYALA
5   THRPHEPHEASPGLYARGPROALAVALPHE
6   ASPGLUALAASPPHEPROALAVALALAVAL
7   TYRLEUTHRGLYALAGLUTYRTHRGLYGLU
8   GLULEUASPSERASPTHRTRPGLNALAGLU
9   LEUHISILEGLUVALPHELEUPROALAGLN
10   VALPROASPSERGLULEUASPALATRPMET
11   GLUSERARGILETYRPROVALMETSERASP
12   ILEPROALALEUSERASPLEUILETHRSER
13   METVALALASERGLYTYRASPTYRARGARG
14   ASPASPASPALAGLYLEUTRPSERSERALA
15   ASPLEUTHRTYRVALILETHRTYRGLUMET

Entity 2, MG_2+ - Mg - 24.305 Da.

1   MG

Samples:

sample_1: gpU, [U-98% 13C; U-98% 15N], 0.8 mM; deutero-Tris, [U-d10], 5 mM; KCl 50 mM; sodium azide 0.02%

condition-1: pH: 7.8; temperature: 293 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
Bruker 600 MHz at York Universitysample_1not availablecondition-1
Varian 800 MHz at NANUC, Edmonton, AB, Canadasample_1not availablecondition-1
Varian 800 MHz at QANUC, Montreal, QC, Canadasample_1not availablecondition-1

Software:

NMRView v5.2 - assignment

nmrPipe - processing

NMR spectrometers:

  • Bruker Avance DRX 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAB35062 BAG76127 BAG77177 BAG77581 BAI23947
EMBL CAQ31258 CAQ97656 CAQ98436 CAR12634 CAR16658
GB AAA96544 AAG55989 AAN81616 ABE06803 ABG69187
REF NP_040591 NP_287377 NP_309666 NP_755046 WP_000352477
SP P03732
AlphaFold P03732

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks